中药芪红合剂对肺间质纤维化模型大鼠肺组织中TGF-β_1表达的影响  

Effect of Qihong Decoction on Transforming Growth Factor-β_1 Expression in Lung Tissues of Model Rats with Pulmonary Fibrosis

作　　者：燕小宁[1] 马华[1] 王青[2] 赵瑞宝[3] 

机构地区：[1]山西医科大学第二医院中医科,山西太原030001 [2]山西大学医院,山西太原030006 [3]山西中医学院,山西太原030024

出　　处：《山西中医学院学报》2007年第2期11-13,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：山西医科大学第二医院2006年新技术新项目(项目编号:2006025)

摘　　要：目的:探讨中药芪红合剂在肺间质纤维化过程中的作用及其机理。方法:采用气管内注射博来霉素A5建立肺间质纤维化的大鼠模型。将40只Wistar大鼠随机分为4组:假手术组、模型组、泼尼松治疗组、芪红合剂治疗组,每组10只。给各组大鼠灌胃相应的生理盐水或药物,术后15d处死各组大鼠,取两肺组织,一部分行石蜡包埋切片,HE染色,观察病理改变,另一部分采用ELISA法定量测定TGF-β1。结果:与假手术组比较,模型组大鼠肺组织有明显的纤维化改变,TGF-β1的含量显著升高(P<0.01);与模型组比较,泼尼松治疗组和芪红合剂治疗组大鼠肺组织中TGF-β1含量显著下降(P<0.01)。结论:中药芪红合剂可能通过降低肺组织中TGF-β1的含量而减轻肺间质损伤及纤维化改变。Objective ：To study the effect of Qihong decoction on rats with pulmonary fibrosis and its mechanism. Methods：Model rats were induced by injecting bleomycin A5 into trachea. 40 Wistar rats were randomized into sham operation control group （given saline）, model group （given saline）, prednisone treatment group, Qihong decoction treatment group （n = 10）. The lung tissues were collected after 15 days treatment. The level of TGF-β1 was measured by ELISA,and the level of fibrosis was observed by HE stain. Results： Compared with sham operation control group, the change of pulmonary fibrosis and the level of TGF-β1 in model group rats were obviously increase（P〈0.01）. Compared with model group,the levels of TGF-β1 in two treatment groups were obviously decreased （P〈0.01）. Conclusion ： Qihong decoction can alleviate lung tissue damage and pulmonary fibrosis through down-regulating TGF-β1 expression.

关 键 词：芪红合剂 TGF-β1 肺间质纤维化 

分 类 号：R563[医药卫生—呼吸系统]