肝肾不足型斑秃患者PBMC中Th1/Th2型细胞因子转录因子T-bet mRNA表达状况  

Study on the Expression of Th1/Th2 Types Gytokines & Transcription Factor T-bet mRNA in the Alopecia Areata Patients with Syndromes of Liver-kiney Deficiency

作　　者：朱培成[1] 禤国维[1] 陈达灿[1] 范瑞强[1] 

机构地区：[1]广东省中医院皮肤科,广东广州510120

出　　处：《中华中医药学刊》2007年第5期937-939,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省科技厅资助项目(2002C3110502)

摘　　要：目的:探讨肝肾不足型斑秃患者外周血单核细胞(PBMC)中Th1/Th2型细胞因子、转录因子T-betmRNA表达状况与斑秃发病的关系。方法:用逆转录—聚合酶链反应(RT-PCR)分别检测8例轻型斑秃、12例重型斑秃(肝肾不足型)患者及10例正常人外周血单一核细胞(PBMC)经PHA刺激后Th1型细胞因子(IFN-γ、IL-12)、Th2型细胞因子IL-10及转录因子T-betmRNA的表达水平。结果:经PHA刺激后轻型斑秃患者PBMC中Th1型细胞因子(IFN-γ、IL-12)基因表达水平轻度升高,与正常对照组比较无显著性差异(P>0.05);Th2型细胞因子(IL-10)基因表达下降,显著低于正常对照组(P<0.05);转录因子T-betmRNA表达水平明显升高,显著高于正常对照组(P<0.05)。重型斑秃患者PBMC中Th1型细胞因子(IFN-γ、IL-12)及转录因子T-bet基因表达不仅显著高于正常对照组(P<0.01),而且还高于轻型斑秃病人(P<0.05);Th2型细胞因子(IL-10)基因表达显著低于正常对照组(P<0.05),与轻型斑秃病人比较无显著性差异。结论:肝肾不足型斑秃患者PBMC中Th1/Th2型细胞因子基因表达失衡,呈现以Th1型细胞因子和转录因子T-bet基因过度表达为特征的Th1型反应,可能参与了斑秃的发病机制,也可能是斑秃肝肾不足证型的微观本质之一。Objective： To investigate the relationship between expression of Th1/Th2 types cytokines （IFN-γ, IL-12/ IL-10） ＆ transcription factor T-bet mRNA in PBMC of the patients with alopecia areata and liver-Kiney deficiency symptoms ; Methods ： Apply RT-PCR technique to detect the mRNA expression of Th1/Th2 types cytokines ＆ transcription factor T-bet in PBMC of the patients with alopecia areata stimulated by with PHA. Results： The mRNA expression of Thl type eytokines in PHA-stimulated PBMC of the with localized alopecia areata patients were increased slightly compared with healthy controls, the expression of transcription factor T-bet mRNA markedly elevated with the healthy controls （ P 〈 0.05）, on the contrary, the mRNA expression of Th2 type cytokines （ IL-10 ） lower significantly than healthy （ P 〈 0.05 ）. However, the mRNA expression of both Thl type cytokines ＆ transcription factor T-bet in PHA-stimulated PBMC of the patients with extensive alopecia areata were markedly increased compared with healthy controls （ P 〈 0.01 ） ＆ localized form （ P 〈 0.05 ）, but the mRNA expression of Th2 type cytokines （ IL-10 ） were lower significantly than healthy controls. （ P 〈 0.05）. Conclusion： there were over-expression of Thl type cytokines and transcription T-bet in the PBMC of alopecia areata especially for extensive form which supported that alopecia areata appears to be a Thl autoimmune condition mediated by over-expression of both IFN-γ ＆ T-bet which also may be the intrinsical essence of patients with deficiency of the liver and kiney.

关 键 词：斑秃 细胞因子 T-BET 基因表达 肝肾不足 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]