愈创营养油对大鼠皮肤缺损创面愈合过程中VGEFmRNA表达的影响  

Effect of Yuchuang Nourished Oil in Full-thickness Skin Wounds of Rats in the Expression of VGEFmRNA

作　　者：张宗学[1] 刘学政[2] 蔡玉文[1] 

机构地区：[1]辽宁中医药大学 [2]辽宁医学院,辽宁锦州121000

出　　处：《中华中医药学刊》2007年第5期1007-1009,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:从分子水平观察愈创营养油对大鼠缺损创面的修复机理。方法:45只背部造成开放性皮缺损创面的大鼠,随机区组分为空白对照组(A组)外敷生理盐水、VEGF的明胶海绵(B组)外敷组外敷、愈创营养油组(C组)涂抹愈创营养油。观察创面愈合时间、毛细血管截面面数密度(NA)及造模后第3、7、14天用RT-PCR的方法检测VEGFmRNA。结果:愈创营养油组愈合时间最短,优于其他各组,与模型组相比有非常显著性差异(P<0.01),且愈创营养油组7天毛细血管截面面数密度对照组相比有非常显著性差异(P<001),在3天和7天VEGFmRNA的表达各组间均有显著性差异,以愈创营养油组表达最好,而14天各组间则没有差异。结论:C组最好,它的作用机制可能是愈创营养油应用后产生的VEGF能明显促进上皮生长、创面愈合。Objective： The study was aimed at investigating the mechanism of Yuchuang nourished Oil in accelerating wound healing into skin restoration at the cell and gene level. Methods： 45 rats were randomized into three groups and 15 rats in each group. After anesthesia injected with ketamine （ 100mg/kg）, under the aseptic condition, a 2.5cm × 2.5cm full thickness wound was made. Group A （saline control group） ,group B（ VEGF treatment group） ,group C（ Yuchuang nourished Oil treatment group）. On day 3,7,14 after wounding, the area and the size of wounds and MDV （ microvessel density）were measured;VEGFmRNA examination was performed with RT - PCR to evaluate the speed and quality of wound healing in different groups. Results ： MDV in the group C （ per cm^2 ） was higher than group B and group A all time points （ P 〈0.01 ） ,and reached its peak in the 7th day. The result of VEGFmRNA is significant difference among every group （ P 〈 0.05） in the 3th and 7th day. And no significant difference was shown in the 14th day. Conclusion： Yuchuang nourished Oil is the best in three groups in accelerating wound healing into skin restoration properly, which may be the role of providing VEGF.

关 键 词：愈创营养油 大鼠 VGEFmRNA 愈合 实验研究 

分 类 号：R264[医药卫生—中医外科学]