电化学发光法测定血清叶酸  

Determination of Serum Folate with Electrochemiluminescence Immunoassay

作　　者：肖路延[1] 王萌[1] 朱明炜[2] 唐志毅[1] 李金明[1] 

机构地区：[1]卫生部北京医院检验科,北京100730 [2]卫生部北京医院外科,北京100730

出　　处：《中国临床营养杂志》2007年第2期75-78,共4页Chinese Journal of Clinical Nutrition

基　　金：首都医学发展科研基金(2002-3041)

摘　　要：目的采用电化学发光法测定血清叶酸含量。方法以三联吡啶钌作标记物,包被叶酸结合蛋白,待测标本中的叶酸与标记叶酸竞争性结合包被好的叶酸结合蛋白,通过外加电场的作用产生化学发光反应,即可测定出血清中叶酸的含量,并将此法测得的结果与放射免疫法测得的结果进行比较分析。结果本法线性范围为1.21～38.80nmol/L,批內和批间的变异系数分别为1.5%和2.4%,回收率为102%。本实验室血清叶酸正常参考值范围为6.2～37.6nmol/L。本法测得的血清叶酸结果与放射免疫法结果比较相关性良好(r=0.97),两种方法测定结果差异无显著性。结论电化学发光法操作简便、灵敏度高、特异性强,结果准确、可靠,适用于临床有关血清叶酸的常规检测和临床研究。Objective To determine the serum folate with electrochemiluminescence immunoassay （ECLIA）. Methods After the serum folate was tied with the ruthenium-labeled folate-binding protein, we supplied a voltage to the electrode and induced chemiluminescent emission. The results were measured by a photomultiplier, and thus the serum folate was determined. ECLIA results were compared with those of radioimmunoassay （RIA）. Results The linear range of ECLIA was 1.21-38.80 nmol/L, and the intra-batch and inter-batch variance coefficiency was 1.5% and 2.4% , respectively. The recovery of folate added to serum sample was 102%. The results determined with ECLIA or RIA had no significant difference. The results were also positively correlated （ r = 0. 97）. Conclusion ECLIA is an accurate, rapid, specific, and suitable approach to measurement serum folate in clinical practice.

关 键 词：叶酸 电化学发光法 

分 类 号：R446.1[医药卫生—诊断学]