毛细管气相色谱法测定烟草中葡萄糖、果糖、蔗糖的含量  

Analysis of glucose, fructose and sucrose in tobacco by capillary gas chromatography

作　　者：张峻松[1] 宣晓泉 唐纲岭[3] 徐如彦 王建民[1] 

机构地区：[1]郑州轻工业学院,郑州东风路5号450002 [2]徐州卷烟厂,徐州221005 [3]郑州烟草研究院,郑州450001

出　　处：《中国烟草学报》2007年第2期17-20,共4页Acta Tabacaria Sinica

基　　金：郑州轻工业学院博士基金项目(No.05014)

摘　　要：建立了一种用毛细管气相色谱法测定不同烟草样品中水溶性葡萄糖、果糖、蔗糖含量的方法。以80％（v／v）乙醇提取烟样中水溶性糖，糖液利用三甲基氯硅烷进行硅醚化反应后，进行毛细管气相色谱分析。结果表明：葡萄糖、果糖和蔗糖的含量与其峰面积具良好的线性（相关系数在0．9995～0．9998），重复性好（RSD〈1．39％），加标回收率在96．19％～99．16％，可以实现对烟草中单糖的分离鉴定。A novel method is proposed for the quantitative determination of water-soluble sugars such as glucose, fructose, and sucrose in tobacco. It included extraction of sugars with 80% （v/v） ethanol, trimethylchlorosilane reaction, and quantitative analysis of capillary gas chromatography. The method shows good linearity with correlation coefficients between 0. 9995 and 0. 9998, and the average recoveries were 99.16 %, 97.98 %, and 96.19 % with good repeatability for glucose, fructose, and sucrose, respectively.

关 键 词：烟草 葡萄糖 果糖 蔗糖 气相色谱 

分 类 号：TS47[农业科学—烟草工业]