HPLC法测定热毒平颗粒中栀子苷的含量  

Determination of geniposide in heat stroke granules by HPLC

作　　者：贺飞[1] 赵杰[1] 李刘辉[1] 刘永[1] 

机构地区：[1]安徽省药物研究所安徽省中药研究与开发重点实验室,安徽合肥230022

出　　处：《安徽医药》2007年第5期412-413,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立热毒平颗粒中栀子苷含量的高效液相色谱法。方法采用C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(12∶88);流速为1.0 ml.min-1;检测波长为238 nm。结果栀子苷在0.128-6.416μg范围内呈线性关系,r=0.999 9。平均加样回收率为99.04%。结论用该法测定热毒平颗粒中栀子苷的含量,操作简便、结果准确、重复性好,可以用于热毒平颗粒的质量控制。Aim To establish a HPLC method for the determination of geniposide in heat stroke granules. Methods The waters ODS C18 （250 mm ×4.6 mm）was used,acetonitrile-water（ 12： 88） as a mobile phase. The flow rate was 1.0 ml · min^-1. The detecting wavelength was 238 nm. Results The linear range of geniposide was 0. 128 - 6. 416 ug, r = 0.9999. The average recovery was 99.04%. Conclusions The method is simple, accurate and well reproducible. It an be used for content determination of geniposide in heat stroke granules.

关 键 词：热毒平颗粒 栀子苷 HPLC 

分 类 号：R286[医药卫生—中药学]