制作标准化血液涂片建议与研究  

Suggestion and Research of the Method for making the standard Blood Smear

作　　者：刘孝武[1] 赵会芹[2] 贺信祥[3] 李金良 刘学军[5] 郭洪海[6] 王英[5] 

机构地区：[1]河北工程大学医学院 [2]邯郸市中心医院,河北邯郸056002 [3]河北医科大学第一医院,河北石家庄050011 [4]邯郸市复兴区中医院,河北邯郸056003 [5]邯郸市中医院,河北邯郸056002 [6]邯郸铁路医院

出　　处：《中国血液流变学杂志》2007年第1期130-131,共2页Chinese Journal of Hemorheology

摘　　要：目的研究标准血液涂片的制备方法。方法制作10°～25°及30°固定角度的推片,在显微镜下观察细胞分布均匀区,进行测量比较,找出符合制备标准血液涂片角度的推片,制备标准血液涂片;与检验技术熟练的人员用传统的制备方法制造的涂片进行比较。结果固定17°或18°的推片,用血量固定在5μL制作的血液涂片血膜长度x和s为4.084,3.996;0.016,0.0102;细胞分布均匀区x和s为3.032,2.976;0.015,0.010。18°固定推片和传统的制备方法比较P<0.001,有显著性差别。结论固定17°或18°的推片,用血量固定在5μL制作的血液涂片符合标准血液涂片。Objective To study the method for making the standard blood smear.Method Some pushing slides whose angle are fixed on 10° to 25° and 30° were made. Under the microscope, the cells uniformly distributed area were observed and the best angle for making standard blood smears were found. These results compared with those which made by the skilled laboratorians using the traditional way.Result To make blood smears with pushing slides angle on 17° and 18° and blood volume on 5 μL, the average length of the membrane x and s were 4.084 and 3.996, 0.016 and 0.0102,repectively.The cells uniformly distributed area x and s were 3.032 and 2.976, 0.015 and 0.010,repectively.There were significant difference between the method using the pushing slides of 18° and the traditional way, P〈0.001 .Conclusion The blood smears which made by the pushing slides whose angle fixed on 17° or 18° , and blood volume fixed on 5μL are standard blood smears.

关 键 词：白细胞分类 标准血液涂片 固定角度 

分 类 号：R446.11[医药卫生—诊断学]