参附注射液对休克大鼠肺损伤的保护作用  

Protective Effect of Shenfu Injection on NF-кB,ICAM-1 and IL-6 Following Shock-associated Lung Injury in Rats

作　　者：陈金仙[1] 陆静[2] 何艳霞[3] 杨引[1] 陈淑琼[1] 

机构地区：[1]广东医学院附属医院麻醉科,广东湛江524001 [2]广东医学院附属医院五官科,广东湛江524001 [3]广东医学院附属医院手术室,广东湛江524001

出　　处：《湖北民族学院学报（医学版）》2007年第1期18-20,共3页Journal of Hubei Minzu University(Medical Edition)

摘　　要：目的探讨休克时肺组织内核转录因子-кB(NF-кB)、细胞间粘附分子-1(ICAM-1)和白细胞介素-6(IL-6)水平的变化及参附注射液对三者的影响。方法健康SD大鼠33只分成3组:手术对照组(C组)、休克组(S组)和参附治疗组(SF组),每组11只。采用股动脉放血复制大鼠休克模型。复苏18h后活杀取肺组织,免疫组织化学法检测肺组织NF-кB活性和ICAM-1水平,ELISA检测大鼠肺组织IL-6水平。结果S组NF-кB活性、ICAM-1和IL-6水平显著高于C组和SF组(P<0.01),且SF组NF-кB活性和IL-6水平高于C组(P<0.05)。结论参附注射液能抑制休克相关性肺损伤NF-кB活性和降低ICAM-1和IL-6水平,减轻休克导致的肺组织损伤。Objective To study NF - kB activity, ICAM - 1 and IL - 6 level following shock - asso- ciated lung injury and the effect of Shenfu injection on them in rats. Methods 33 SD rats were randomized into three groups each consisting of 11 rats： Sham- operated control group（ C group） ,shock group（ S group）, Shenfu injection group（ SF group） ,shock models were induced by hemorrhaged from the femoral artery to the mean arterial pressure of 35 mmHg,1 hour later,S group were resuscitated with twofold volume lactated Ringer＇s solution, whereas SF group with Shenfu injection. The activity of NF - lB and the level of ICAM - 1 in lung were detected by immunohistochemical technique, and the level of IL -6 in lung were measured by ELISA method. Results The activity of NF -kB ,the level of ICAM -1 and IL-6 in S group were significantly higher than those in C and SF group（P 〈 0.01 ）,and the activity of NF -kB, the level of IL -6 of lung tissue in SF group were higher than those in C group （P 〈0.05）. Conclu sion Shenfu injection can inhibit the activity of NF - kB, decrease ICAM - 1 and I L - 6 level ,alleviate shock- associated lung injury.

关 键 词：参附注射液 休克 肺损伤 细胞因子 

分 类 号：R285.5[医药卫生—中药学] R332.2[医药卫生—中医学]