卡介苗免疫对大鼠抗结核发病和T淋巴细胞亚群的影响  

Impact of BCG Vaccine on Anti-tuberculosis and CD4^+ CD8^+ Double Positive T Lymphocyte Subset in Rats

作　　者：雷建平[1] 彭燕[2] 熊国亮[1] 张玉珍[3] 周军[1] 刘翼军[1] 邢娟娟[2] 李光明[1] 雷铭宙[1] 袁小亮[1] 涂荣耀[1] 涂少华[1] 

机构地区：[1]江西省胸科医院,南昌330006 [2]南昌大学第一附属医院流式细胞室 [3]南昌大学医学院动物科学部

出　　处：《中国预防医学杂志》2007年第2期81-84,共4页Chinese Preventive Medicine

基　　金：江西省社会发展攻关重点基金(2004)

摘　　要：目的探讨卡介苗(BCG)对大鼠结核感染及CD4+CD8+双阳(DP)T细胞的影响。方法60只结核菌素纯化蛋白衍生物(PPD)皮试阴性SD大鼠随机分成:①BCG免疫组;②BCG免疫后结核分枝杆菌(Mtb)攻击(BCG-TB)组;③Mtb攻击后不化疗(TB)组。BCG组、BCG-TB组于观察开始接种BCG。BCG-TB组、TB组于3个月末接受Mtb攻击。比较全部大鼠接种Mtb后的死亡率;实验开始和其后3、4、5、6、8个月末检测外周血中T细胞亚群。结果BCG组、BCG-TB组无死亡;TB组感染后5个月内死亡率45%(9/20)。TB组死亡率高于BCG组和BCG-TB组,P<0.01。实验开始时3个组组间对应参数比较差异均无统计学意义(P>0.05);3个月末BCG组、BCG-TB组之间各对应参数比较差异均无统计学意义(P>0.05);BCG组和BCG-TB组第3个月末与接种前比较,CD8+、DP升高,且显著高于TB组(Mtb干预之前),P<0.01或P<0.05。BCG组DP细胞于接种BCG后第6个月后开始回落,但仍显著高于接种前;BCG-TB组于Mtb攻击1个月后CD8+、DP继续升高,观察的第8个月CD8+、DP回落,但仍显著高于BCG接种前;TB组Mtb攻击后1个月末CD8+、DP有升高但低于BCG组和BCG-TB组,同时伴有CD3+、CD4+降低,差异均有统计学意义,P<0.05或P<0.01,此后维持在相近水平。Mtb攻击2个月末BCG-TB组DP高于BCG组和TB组,P<0.05或P<0.01。Mtb攻击5个月后,3个组间DP差异无统计学意义(P>0.05)。结论BCG预防接种可保护Mtb感染宿主免于发病和死亡,增强抗结核保护性细胞免疫力表现之一为DP细胞升高,DP细胞是抗结核保护性免疫相关T细胞。DP细胞的形成与CD4+SP细胞上CD8+抗原表达有关。Objective To investigate the effects of Bccillus Calmette Guerin （BCG） vaccine on anti - tuberculosis and CD4^＋ CD8^＋ double positive （DP） T lymphocytes. Methods 60 SD rats with negative PPD skin tests were randomly divided into the following 3 groups： （1）BCG group; （2）BCG- TB group; Rats were infected by mycobacerium tuberculosis （Mtb） 3 months after the rats received BCG vaccine; （3）TB （tuberculosis） group.Mortality of each group was measured.Peripheral CD3^＋ , CD4^＋ , CD8^＋ and DPT lymphocytes were measured at the beginning and the end of 3rd. 4th. 5th. 6th. 8th month of the experiment. Results （1）Mortality of TB group is 45% （9/20）, higher than that of the other two groups （0/40）; （2）CD8^＋ and DP T lymphocyte subset of BCG group increased significandy at the end of 3rd month; （3）Although ratio of CD4/CD8 （without CD4^＋ ） decreased, peripheral CD8^＋ and DP T lymphocytes increased significantly at the end of 1st month after rats were infected with Mtb; （4）Rafio of CD4/CD8 （may with CD4^＋ or CD3^＋ ） decreased while CD8^＋ . DP T lymphocytos increased significantly in TB group. Conclusion （1）DP T cell conducts protective immunological effects against tuberculosis; （2）Expression of CD8^＋ on CD4^＋ SP T cell may correlate with the comforming of DP T cell.

关 键 词：结核病 细胞免疫学 CD4^+CD8^+双阳(DP)T细胞 BCG 

分 类 号：R392.1[医药卫生—免疫学]