低强度He-Ne激光对红细胞胞浆内游离钙离子浓度的影响  

Effects of Low Intensity He-Ne Laser on Cytosolic Free Calcium Concentration in Human Erythrocyte

作　　者：郑莹[1] 郭周义[1] 赵燕平[2] 崔艳红[1] 刘颂豪[2] 

机构地区：[1]华南师范大学激光生命科学教育部重点实验室 [2]华南师范大学信息光电子科技学院光子中医学实验室,广东广州510631

出　　处：《激光生物学报》2007年第2期127-134,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金(60278012);广东省自然科学基金(No.06025080;No.015012)

摘　　要：低强度He-Ne激光对红细胞变形性的影响已得到广泛的认可和应用,但其具体调节机制尚不明确,通过研究低强度He-Ne激光对红细胞胞浆内钙离子浓度的影响,探讨其对红细胞变形性影响的机制。采用A23187处理过的红细胞,分别用5 mW和9 mW激光照射后,观察红细胞胞浆内钙离子浓度变化。结果显示:低强度He-Ne激光照射后的红细胞与无照射组的红细胞相比,红细胞胞浆内钙离子浓度显著降低,但在本实验中钙离子浓度的降低与激光照射剂量无显著相关。由此得出结论:降低红细胞胞浆内钙离子浓度可能是低强度He-Ne激光调节红细胞变形性的重要机制。The effect of low intensity He-Ne laser have already been ratified and utilized. But the mechanism is still in equivocation. The purposes of studying is to find out this mechanism through studying the effects of low intensity He-Ne laser on human erythrocyte which is irradiated by 5mW and 9mW He-Ne laser after treated with A23187. The results are ： after irradiation, the concentration of free Ca^2＋ in erythrocytes is lower than that is not irradiated. Low intensity He-Ne laser can decrease the cytosolic free calcium concentration of erythrocytes. But no does relation is showed in this experiment. The conclusion is that low intensity He-Ne laser may increase human erythrocyt deformability by decreasing the cytosolic free calcium concentration, which is effective to improve the microcirculation of patients with hypertension whose initial cytosolic free calcium concentration is higher than normal.

关 键 词：低强度激光 钙离子 红细胞 变形机制 

分 类 号：Q631[生物学—生物物理学] Q274