荧光相关谱测量技术研究  

Studies on a New Technique of Fluorescence Correlation Spectroscopy

作　　者：梁丽芳[1] 邢达[1] 陈同生[1] 裴沂辉[1] 

机构地区：[1]华南师范大学激光生命科学研究所暨激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2007年第2期243-248,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金面上项目(60378043;30470494);广东省自然科学基金项目(015012;04010394);广东省科技计划项目(2004B10401011)资助

摘　　要：荧光相关谱(fluorescence correlation spectroscopy,FCS)是对处于热平衡态条件下的荧光分子发出的荧光强度涨落进行时间相关处理的一种单分子检测方法,能够直接测量分子在溶液里的扩散系数和浓度。影响FCS测量扩散系数准确性的因素有分子量子效率,测量时间,样本折射率和温度偏差等。用FCS分别测量溶有荧光染料罗丹明6G(rhodamine 6G,Rh.6G)和青色素Cy5甘油水溶液的粘滞系数,实验结果表明:荧光分子的量子效率是影响测量准确性的重要因素,要求其每秒发射的光子数目(photon counts per second,cps)至少达到1 000(photons/s)。Fluorescence correlation spectroscopy （FCS）, a kind of single molecule technique, performs temporally correlative analysis of spontaneous intensity fluctuation of fluorescence from thermal equilibrium with direct measurements of the diffusion coefficient and the concentration of fluorescence molecules in aqueous solution. Some factors, such as the quantum coefficient, the measurement time, the refractive index of the sample and the temperature deviation, make effects on the measurement accuracy. In the thesis, FCS is used to measure the viscosity of the glycerin-water solution with Rhodamine 6G and Cy5, respectively and the experiment results show that the quantum coefficient plays an important role in the measurement accuracy with 1000 photons/sec as the minimal level.

关 键 词：荧光相关谱 单分子检测 粘滞系数 量子效率 折射率 

分 类 号：Q-331[生物学]