牛血清白蛋白在超薄纳米二氧化钛膜表面的印迹与吸附  

Molecular Imprinting and Adsorption of Bovine Serum Albumin on Nanocrystalline Titania Films

作　　者：谢亚林[1] 司士辉[1] 杨政鹏[1] 李赛[1] 彭芳[1] 

机构地区：[1]中南大学化学化工学院,长沙410083

出　　处：《分析化学》2007年第4期555-558,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No20475065)

摘　　要：基于溶胶凝胶分子印迹方法，以溶胶二氧化钛TiO2为基质印迹了牛血清白蛋白分子。用1％的NaOH溶液可有效地除去纳米TiO2印迹膜中的模板分子。采用石英晶体微天平现场技术，研究了牛血清白蛋白在超薄纳米TiO2膜表面的吸附行为。研究表明，牛血清白蛋白在印迹膜和非印迹膜上的吸附量都随溶液浓度增加而增大，印迹膜具有吸附的特异性和可再生性，其吸附量是非印迹膜的3～5倍；在非印迹膜上的吸附符合Langmuir吸附模型，而在印迹膜上的吸附符合allosteric吸附模型；牛血清白蛋白在非印迹膜上的吸附量先随pH升高而增大，当pH为5左右时达到最大值，随后吸附量又随pH的增大而减小；而在印迹膜上其吸附量仅随pH增大而增大。The bovine serum albumin （BSA） imprinted TiO2 film was synthesized via surface sol-gel process, using BSA as template and TiO2 sol as imprinted matrix. Appropriate template cavities in the TiO2 sol-gel film were formed after the template molecules were removed on treatment with 1% NaOH solution. In-situ technique of quartz crystal microbalance （QCM） was employed to study the molecular imprinting behavior of BSA on nanocrystalline titania films. The imprinted film showed selectivity recognition for BSA as compared to the other proteins. The amount of adsorption increased with the increasing of BSA concentration both on imprinted film and non-imprinted film. The adsorption amount increased with the increasing of pH on imprinted film and appeared a maximum value at pH 5 on non-imprinted film. The binding behavior of BSA onto non-imprinted film fitted into the Langmuir model while onto imprinted film fitted into allosteric model.

关 键 词：牛血清白蛋白 分子印迹 石英晶体微天平 纳米二氧化钛 

分 类 号：Q51-3[生物学—生物化学]