奈替米星-刚果红分光光度法测定奈替米星  

The Fading Color Reaction of Netilmicin with Congo Red and Its Application to the Spectrophotometric Determination of Netilimicin

作　　者：江虹[1] 湛海粼 何树华[1] 刘艳[1] 

机构地区：[1]涪陵师范学院化学及环境科学系,重庆408003 [2]涪陵广播电视大学,重庆408000

出　　处：《分析化学》2007年第4期575-578,共4页Chinese Journal of Analytical Chemistry

基　　金：重庆市教委科技基金资助项目(NoKJ051304)

摘　　要：在pH4.5-6.0和pH1.9-3.1的酸性条件下,奈替米星（NTM）与刚果红（CR）反应,分别生成红色和蓝色离子缔合物,使刚果红红色溶液褪色。前者最大褪色波长位于500nm处,表观摩尔吸光系数（ε）为2.55×10^4Lmol^-1cm^-1;奈替米星在0.04-9.5mg/L浓度范围内,遵从朗伯比尔定律。后果者也使奈替米星在一定浓度范围内遵从朗伯比尔定律,但灵敏度不及前者。本文探讨了适宜的反应条件、主要分析化学性质及两种不同酸度范围内的络合比。所拟定的方法简便、快速,用于市售奈替米星药物及人尿、人血清中奈替米星含量的测定,结果满意。In the acid condition of pH 4.5 - 6.0, netilmicin （NTM） reacts with Congo red （CR） to form red ion association complexes and bring to the red solution fading of Congo red. The maximum fading wavelengths were located 500 nm, the apparent molar absorptivities （ε） was 2.55×10^4L mol^-1cm^-1）. The concentration of netilmicin obeys Lombard Beer＇s law in 0.04 - 9.50 mg/L. In the acid condition of pH 1.9 - 3.1, netilmicin （NTM） reacts with CR to form blue ion association complexes and bring to the blue solution fading of Congo red. Netilmicin also obeys Lombard Beer＇s law in definite concentration bound, but preceding sensitive degree higher.

关 键 词：奈替米星 刚果红 分光光度法 含量测定 氨基糖苷类抗生素 

分 类 号：O657.3[理学—分析化学]