结晶紫在鳗鲡组织中的代谢和消除规律研究  

Studies on the Metabolism and Depletion Principles of Crystal Violet in Eel Tissues

作　　者：蔡春平[1] 林岗[2] 刘正才[1] 方宇[1] 李小晶[1] 苏芝娇[1] 王丹红[1] 

机构地区：[1]福建出入境检验检疫局技术中心,福建福州350003 [2]福建师范大学生命科学学院,福建福州350007

出　　处：《中国兽药杂志》2007年第4期15-18,共4页Chinese Journal of Veterinary Drug

基　　金：福建省自然科学基金资助(B0410029)

摘　　要：在水温18—21℃，结晶紫浓度为0．1mg／L的条件下，对鳗鲡进行24h药浴后，采用高效液相色谱-串联质谱仪，对鳗鲡肌肉组织中的结晶紫和隐色结晶紫含量进行连续56d的监测。结果表明：鳗鲡肌肉组织中结晶紫的最高浓度出现在给药后12h；在整个监测过程中，肌肉组织中结晶紫的含量都较低（0～3．91μg／kg）；药浴336h后，结晶紫未检出。结晶紫在鳗鲡组织中代谢为隐色结晶紫的速度很快，药浴后3h即可测得隐色结晶紫，且含量明显高于结晶紫。隐色结晶紫的最高含量出现在药浴后的72h。在监测的第56天，隐色结晶紫的含量仍达19．5μg／kg。可见，隐色结晶紫在鳗鲡肌肉组织中的消除过程长，是鳗鲡机体肌肉组织中药物存在的主要方式。The eels were exposed to crystal violet （CV） bath water of 0.1 mg/L for 24 h at a water temperature varying between 18 -21 ℃. After treatment, the residues of crystal violet and leuco crystal violet （LCV） in eel tissues were analysed by HPLC-MS. The highest concentration of CV was found at 12 h after the treatment. During the experiment, the concentrations of CV were relatively low. At 336 h after treatment, residues of CV were not detectable. CV in muscle tissues of eels was rapidly metabolized to its leuco form, LCV, which was detectable only after 3 h of exposure, and the concentration of LCV was higher than that of CV. The highest concentration of LCV was found at 72 h after the treatment. At 56 d after exposure, the concentration of LCV in eel tissues still reached 19.5 μg/kg, which indicated that LCV was the main form in muscle tissues, and the depletion of LCV in eels was a long-term process.

关 键 词：鳗鲡 结晶紫 残留 代谢 消除规律 

分 类 号：R917[医药卫生—药物分析学]