奶牛血液中蓖麻碱含量的HPLC检测  

Determination of Ricinine in Dairy Cow Blood by HPLC

作　　者：张显华[1] 张学富[2] 张翠艳[1] 窦亚平 

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]内蒙古民族大学分析测试中心,内蒙古通辽028000 [3]通辽市科尔沁区兽医工作站,内蒙古通辽028000

出　　处：《中国兽药杂志》2007年第4期25-28,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了血液中蓖麻碱残留量的高效液相色谱（HPLC）检测法。用三氯甲烷提取蓖麻碱，除去蛋白质和脂肪后，用HPLC法对其进行测定。采用Hypersil ODSC C18色谱柱（250mm×4．6mm，25μm），水-乙腈（9：1，V/V）为流动相，流速0．8mL／min，检测波长为220nm。结果显示，在0．26—5．2μg范围内，蓖麻碱峰面积与质量的线性关系良好，回归方程为y=1．57×10^4 x-4．93×10^5，r=0．999，RSD=3．82％。牛血中蓖麻碱平均回收率为72．1％，RSD为2．54％（n=5）。饲喂蓖麻粕的牛血液中蓖麻碱的含量为0．512μg／mL。A HPLC method for determination of ricinine in the blood was established. Ricinine was extracted by chloroform, separated with Hypersil ODS Cls （ 250mm × 4.6mm, 25 μm） chromatogram column and acetonitrilewater（9： 1, V/V） as liquid phage that the flow rate was 0.8 mL/min, determined with diode array detector and ultraviolet detector at 220 nm of the wavelength. Results showed that there was a good linear relation in the range of 0.26-5.2 μg ricinine, the equation of recovery rate was y = 1.57 - 10^4x -4.93 × 10^5 （ r =0. 999 ）, RSD was 3.82%. Average recovery rate of ricinine in cow blood was 72.1% , RSD was 2.54% （ n = 5 ）. The content of ricinine in blood from the dairy cow fed with castor bean meal was 0.512 μg/mL.

关 键 词：蓖麻碱 高效液相色谱法 奶牛血 检测 

分 类 号：S859.81[农业科学—临床兽医学]