生物絮凝剂ZL 5-2的絮凝特性研究  

Study on the flocculating characterization of bioflocculant ZL 5-2

作　　者：李强[1] 王元秀[2] 矫强[2] 陈明[1] 张玉臻[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100 [2]济南大学化学化工学院,山东济南250022

出　　处：《化学工程》2007年第4期46-48,52,共4页Chemical Engineering(China)

基　　金：国家自然科学基金项目(30270046);济南大学博士基金项目(B0602)

摘　　要：ZL 5-2是土壤杆菌M-503产生的一种新型生物絮凝剂。经组分分析和薄层层析分析,表明ZL 5-2是一种含有少量蛋白的多糖类物质。通过对絮凝活性影响因素的研究,发现Ca2+,Ba2+,Mg2+,Mn2+及A l3+对絮凝活性具有促进作用,其中Ca2+助凝效果最好;ZL 5-2在pH值为6—12下保持很高絮凝活性,耐碱性良好;ZL 5-2在4—121℃下保留了绝大部分的絮凝活性,表现出良好的热稳定性;胃蛋白酶、胰蛋白酶、三氯乙酸及α-淀粉酶、纤维素酶、-β1,4葡聚糖酶等糖苷酶作用于ZL 5-2,对絮凝活性均无明显影响;-β1,3葡聚糖酶可降解ZL 5-2中的多糖物质,但对絮凝活性无明显影响。A novel bioflocculant ZL 5-2 was produced by Agrobacterium sp. M-503. The main component of ZL 5- 2, i.e. , a kind of polysaccharide, was detected to be mainly consisting of glucose through chemical composition and TLC analysis. It shows that some metal ions, such as Ca^2＋ , Ba^2＋ , Al^3＋ , Mg^2＋ and Mn^2＋ can improve its flocculating ability, with Ca^2＋ presenting the best effects. The bioflocculant is applied effectively in a pH range of 6-12 and temperature range of 4-121 ℃. ZL 5-2 is not biodegradable by pepsin, trypsin, trichloroacetic acid, α- amylase, cellulase and 8-1,4 dextranase. Although 8-1,3 dextranase can degrade the polysaccharide component of the ZL 5-2, no significant effect on the flocculating ability of ZL 5-2 is found by all of these glycosidase and other effectors.

关 键 词：生物絮凝剂 絮凝活性 糖苷酶 

分 类 号：X703[环境科学与工程—环境工程]