美伐他汀产生菌的原生质体诱变育种  

Slecetion of High Mevasatin-producing Strain through Mutation of Protoplast Induced by UV Radiation

作　　者：郄丽萍[1] 张玉莲[1] 陈丽华[1] 代明伟[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《河北化工》2007年第4期32-33,共2页Hebei Chemical Industry

摘　　要：以美伐他汀产生菌SIPI-8915为出发菌,研究了影响原生质体形成和再生的各种因素并选出最佳条件,其再生频率达到23.5%;并对原生质体进行紫外诱变育种,筛选到变异株。该菌株摇瓶发酵效价比对照提高30%,传代稳定好。o select a high mevasatin-producing strain through protoplast mutation induced by UV radiation, factors affecting protoplast formation and regeneration were studied ;the protoplast were obtained and the regeneration frequency was 23.5%, the protoplast of SIPI-8915 were induce by UV radiation ;the mutants were screened;a high mevasatin-producing strain was obtained, whose mevasatin output increased by 30% compared with the starting strain.

关 键 词：美伐他汀 原生质体 诱变 筛选 

分 类 号：Q933[生物学—微生物学]