无菌性脑膜炎患者脑脊液中肠道病毒RNA的检测分析  

Analysis on the detection of enterovirus RNA in cerebrospinal fluid from patients with aseptic meningitis

作　　者：关慧臻[1] 杨桂梅[1] 薛承岩[2] 李桂洁[1] 

机构地区：[1]承德医学院附属医院检验科,河北承德067000 [2]承德医学院附属医院中心实验室

出　　处：《华北煤炭医学院学报》2007年第3期294-295,共2页Journal of North China Coal Medical College

基　　金："2006年承德市科学技术研究与发展计划"项目(项目编号:200622020)

摘　　要：①目的分析脑脊液中肠道病毒(EV)RNA的检测结果,了解所检测的RNA病毒在脑膜炎中的致病作用。②方法检测脑脊液标本EV RNA应用EV通用引物聚合酶链反应方法。③结果无菌性脑膜炎组EV RNA的阳性率为31.1%(47/151),其中儿童组42.6%(26/61)、成人组23.3%(21/90),两组阳性结果的差别具有显著性(P<0.05)。37例对照组未检出EV RNA。④结论EV是导致无菌性脑膜炎的重要病原体。聚合酶链反应方法可以用于脑膜炎的临床病原学诊断。Objective To analyze the detected results of enterovirus RNA in cerebrospinalfluid and understand the relationship between enterovirus infection and meningitis. Methods Enterovirus RNA was detected by the general primer - polymerase chain reaction in cerebrospinal fluid. Results The positive rate of enterovirus RNA in aseptic meningitis group was 31. 1% （47/151）, in which children group was 42.6% （26/61） and adult group was 23.3% （21/90） （P 〈0.05 ） , while enterovirus RNA was not examined out in 37 control cases. Conclusions The enterovirus was the important pathogens which cause aseptic meningitis, polymerase chain reaction was a useful tool for the clinic etiologic diagnosis of meningitis.

关 键 词：脑膜炎 肠道 病毒 脑脊液 RNA 

分 类 号：R446.14[医药卫生—诊断学]