壳聚糖磁性纳米粒子药物组装及其缓释性能  

Magnetic chitosan-5Fu-nanoparticles conjugation and drug releasing properties investigation

作　　者：阮溦 王亚东[2] 阮建明[2] 

机构地区：[1]中南大学湘雅医学院,湖南长沙410052 [2]中南大学粉末冶金研究院,湖南长沙410083

出　　处：《中国医学工程》2007年第2期113-117,121,共6页China Medical Engineering

基　　金：国家863课题(2001AA218011)

摘　　要：目的组装癌症治疗药物五氟尿嘧啶,构成功能性磁性壳聚糖纳米粒子药物载体。方法用化学共沉淀法制备了Fe3O4纳米颗粒,经优化胶体化学反应技术采用壳聚糖改性。经激光粒度仪、透射电子显微镜,红外光谱以及紫外-可见光谱进行形貌与粒度研究分析。结果其中磁性Fe3O4颗粒,壳聚糖改性磁性Fe3O4,以及载药(五氟尿嘧啶,5-Fu)壳聚糖改性磁性Fe3O4纳米颗粒的粒径分别为8￣12nm,12￣15nm和20￣30nm,颗粒形状呈规则球型;壳聚糖改性作用和改性后纳米磁性Fe3O4载药效果明显。壳聚糖-五氟尿嘧啶(5-Fu)和壳聚糖改性磁性Fe3O4纳米颗粒载药量分别达到15.8%和9.3%。结论上述纳米药物载体在磷酸盐缓冲液中均表现出明显的缓释特性。[Objective] To assemble the medication 5-Fu for the treatment of cancer. [Methods] Fe3O4 nanoparticles as a kernel, 5-Fu, Fe3O4 and chitosan aqueous slurry were mixed by an appropriate proportion to produce the magnetic chitosan-5-Fu composed nanoparticles. Magnetes （Fe3O4） powder was treated by chemical method and transformed into nanometer size aqueous slurry. After Chitosan and Fe3O4 aqueous slurry were mixed and prepared by a optimized condition, the morphology and size of the magnetic chitosan-5-Fu composed nanoparticles were observed by TEM. Drug carried capacity and releasing properties in the phosphate buffer solution were investigated by UV-vis spectrum. [Results] Transmission electron microscope （TEM） shown the magnetes Fe3O4, the magnetic chitosan nanoparitcles and the magnetic chitosan 5-Fu shaped spherical form with a size rang of 8-12 nm, 12- 15 nm and 20-30 nm. The results tested by UV-Vis spectroscope to study the magnetic response displayed that magnetic chitosan nanoparticles had good magnetic properties. The observations shown that the magnetic chitosan- 5-Fu nanoparticles had spherical shape with a size range of 20-30 nm. The research results indicated that the carried capacity of 5-Fu-chitosan and magnetic chitosan-5-Fu nanoparticles were 15.8% and 9.3% respectively. [Conclusions] Drug releasing rate decreased with time lasting in above buffer.

关 键 词：纳米技术 表面改性与药物组装 缓释性能 

分 类 号：R944.9[医药卫生—药剂学]