烟草内生青枯病拮抗细菌的筛选和初步鉴定  

Selection and Preliminary Identification of Tobacco Antagonistic Endophytic Bacteria against Ralstonia Solanacearum

作　　者：彭细桥[1] 刘红艳[2] 罗宽[3] 邓正平 

机构地区：[1]湖南省烟草公司郴州市公司烟科所,湖南郴州423000 [2]湖南省茶叶研究所,长沙410125 [3]湖南农业大学,长沙410128

出　　处：《中国烟草科学》2007年第2期38-40,共3页Chinese Tobacco Science

基　　金：湖南省烟草专卖局基金资助项目(HN-02-006)

摘　　要：为了获得防治烟草青枯病新途径,从湖南桂阳烟草青枯病重病区采集的健株上,共分离到内生菌株160个。经对峙培养法测定,32个菌株对烟草青枯菌有拮抗作用,其中H-1、H-9和H-11有强拮抗作用,抑菌圈分别为3.0、3.0、4.5 mm。根据其形态特征和生理生化性状,初步鉴定内生菌H-1为枯草芽孢杆菌,H-9和H-11为短芽孢杆菌。One hundred and sixty endophytic bacteria strains were isolated from health tobacco plants in Guiyang county of Hunan province in disease severely infested plots. Thirty two, in particular three endophytic bacteria strains showed relatively strong antagonistic actions against Ralstonia solanacearum. Culturing these three bacteria and Ralstonia solanacearum together, the results showed that three endophytic bacteria H- 1, H-9, and H- 11 had the best antagonistic effects, and bacteriostnsis diameters were 3.0, 3.0, and 4.5mm： respectively. With regard to morphologic, physiological, and biochemical characterics, strain H-1, H-9, and H-11 were identified. The results showed that H-1 was Bacillus subtilis. H-9 and H- 11 were Bacillus brevis. We also identified that they were all endophyfic bacteria in tobacco.

关 键 词：烟草青枯病 拮抗细菌 定殖 鉴定 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]