SH7对烟草青枯病的抑菌、防病作用及其抑菌物质的初步研究  

A Preliminary Study on Inhibition Activity and Antimicrobial Substance of Antagonistic Bacteria SH7 to Tobacco Wilt

作　　者：王静[1] 赵廷昌[1] 李玉高[2] 孔凡玉[3] 何月秋[4] 张成省[3] 刘建华[5] 刘伟成[5] 

机构地区：[1]中国农业科学院植物保护研究所,北京100094 [2]山东省烟草公司临沂市公司,山东临沂276511 [3]中国农业科学院烟草研究所,青岛266101 [4]云南农业大学植物保护学院,昆明650201 [5]北京市农林科学研究院植物保护环境保护研究所,北京100089

出　　处：《中国烟草科学》2007年第2期41-44,共4页Chinese Tobacco Science

基　　金：国家烟草专卖局资助项目(110200201008);北京市科技计划项目(D0706005000091)

摘　　要：研究了生防细菌菌株SH7及其除菌发酵液对烟草青枯病菌(Ralstonia solanacearum)的抑菌和防病作用,并对其产生的抑菌物质进行了粗提。结果显示,该菌株对R.solanacearum有很强的抑菌作用,且其除菌体发酵液对烟草青枯病的防效较好。将SH7无菌发酵液、无菌发酵液与青枯病菌混合30 min施入土壤29 d后,两种处理与对照相比,防效分别达到65.99%和53.2%;SH7菌株产生的抑菌物质是SH7除菌发酵液防病的主要机制;SH7分泌的抑菌物质可以被硫酸铵沉淀,初步认为该抑菌物质是蛋白类物质。The inhibition activity of the strain SH7 to tobacco wilt （Ralstonia solanacearum） , isolated from the rhizopheres of tobacco plants, was studied. Disease control with SH7 bacterium-free ferment was carried out. We extracted its antimicrobial substance, too. The results indicated that the strain SH7 was strongly antagonistic to Ralstonia solanaceatum. Compared to those of CK in 29d after treatment, the control effects of SH7 bacterium-free ferment, which included two treatments （watering roots with SH7 bacterium-free ferment and with the 30 rain mixed suspension of SH7 bacterium-free ferment and Ralstonia solanacearum） were 65.9% and 53.2%, respectively. It implied that the excretion of SH7 is a very important antagonisfism. This antimicrobial substance was purified by ammonium sulfate with 70% saturation, and we pre-concluded that it belongs to a kind of protenlike substances.

关 键 词：烟草青枯病 抑菌物质 生物防治 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]