甲状腺功能亢进肝阳上亢证大鼠下丘脑组织蛋白质差异表达的观察  

Observation of Differential Protein of Hypothalamus in the Hyperthyroidlsm Rats with Hyperactivity of Liver-Yang

作　　者：周凌燕[1] 陈泽奇[1] 李炜[1] 李智[1] 颜永平[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国中医药信息杂志》2007年第5期18-20,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30472115)

摘　　要：目的利用蛋白质组学方法,观察甲状腺功能亢进(简称"甲亢")肝阳上亢证大鼠与正常大鼠下丘脑组织的双向凝胶蛋白图谱,寻找差异表达的蛋白质。方法左甲状腺素钠(L-T4)和附子汤灌胃造成甲亢肝阳上亢证模型,应用蛋白质组学技术比较模型组与正常组中下丘脑的差异蛋白质,结合生物信息学对其进行分析鉴定。结果两组蛋白斑点总体分布相似,主要分布在等电点pI 3～8,分子量Mr 14.4～75kD;分析发现,模型组与正常组相比有18个蛋白质上调和24个蛋白质下调,进一步质谱鉴定,其中7个蛋白质分别为NSFL1辅助因子、硫氧还蛋白、泛素羧基末端水解酶同工酶L1、血小板活化因子乙酰基水解酶IB—γ亚单位、谷氨酸脱氢酶前体、二氢嘧啶酶相关蛋白2和微管蛋白。结论模型组与正常组相比,有18个蛋白质点上调,有24个蛋白质点下调,经质谱鉴定了其中部分蛋白质.蛋白质的变化可能与甲亢肝阳上亢证的形成密切相关。Objective Utilization of proteome method, by observing differences on protein expression of hypothalamus in the hyperthyroidism rats with hyperactivity of liver-yang and normal rats, to search the differential protein. Metheds Models of animal were prepared by ip L-T4 and Fuzi Decoction. Both the quantitative and qualitative changes of the protein expression were compared between model group and treated group by protein technique. Results Proteins were mainly displayed at range of pl 3～8, Mr （14.4～ 75）kD. 18 protein increased and 24 protein decreased in model group compared with treated group, included Thioredoxin, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Platelet-activating factor acetylhydrolase IB gamma subunit, glutamate dehydrogenase, dihydropyrirrddinase-related protein 2, NSFL1 cofactor p47, Tubulin beta 5. Conclusions There were 18 spots high expressed and 24 spots low expressed in the hyperthyroidism with hyperactivity of liver-yang, that maybe related with the develepment of the hyperthyroidism with hyperactivity of liver-yang.

关 键 词：甲状腺功能亢进 肝阳上亢证 下丘脑 蛋白质组学 动物模型 

分 类 号：R289.11[医药卫生—方剂学]