环丙沙星与DNA相互作用的电化学研究  

Electrochemical studies of ciprofloxacin and its interaction with DNA

作　　者：时巧翠[1] 王素芬[1] 朱斌 季敏 

机构地区：[1]浙江大学化学系,浙江杭州310028 [2]浙江公安高等专科学校,浙江杭州310053

出　　处：《浙江大学学报（理学版）》2007年第3期330-334,共5页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(29975024;20275034)

摘　　要：研究了环丙沙星(CFX)在自制的浸蜡石墨电极上的电化学特性,示差脉冲伏安法发现CFX在0.88 V处有一明显的氧化峰,DNA的加入使这一氧化峰明显减弱.采用所推导的电化学公式对实验数据进行了非线性拟合,同时获得了CFX与DNA的结合常数(K=1.36105(mol/L)-1)和结合位点数(s=1.94),并探讨了CFX与小牛胸腺DNA的结合机理.The electrochemical behavior of ciprofloxacin （CFX） and its interaction with calf thymus DNA （ctDNA） were studied by differential pulse voltammetry on a carbon paraffined electrode. CFX showed a well-defined oxidative peak at ＋0. 88 V. As a result of reaction with ctDNA, the oxidative peak of CFX decreased apparently. According to the electrochemical equation deduced in this paper, the binding constant of 1.36×10^5 （mol/L） ^-1 and the binding size of 1.94 （base pairs） of CFX with ctDNA were obtained by nonlinear fit analysis of electrochemical data. The mechanism of the interaction was explored.

关 键 词：环丙沙星 DNA 电化学方法 相互作用 

分 类 号：O657.1[理学—分析化学]