犬狂犬病毒抗体ELISA检测方法的建立  

Establishment of ELISA in detecting experimental canine rabies virus antibody and reagent standardization

作　　者：杨文祥[1] 徐国景[1] 唐利军[1] 肖红雨[1] 孙凡中[1] 张金明[1] 林乔[1] 易平[1] 龚镇奎[1] 

机构地区：[1]湖北省疾病预防控制中心,武汉430079

出　　处：《公共卫生与预防医学》2007年第2期5-8,共4页Journal of Public Health and Preventive Medicine

基　　金：国家科技部重点科研课题(项目编号2002DEA20023)

摘　　要：目的建立犬狂犬病毒抗体ELISA检测方法。方法采用RV-CTN株感染Vero细胞制备狂犬病毒抗原,并经浓缩和纯化后,分别建立间接ELISA和竞争ELISA方法。结果经初步质量鉴定,特异性10份阳性,20份阴性,符合率均达100%,间接ELISA的灵敏度达1∶640,竞争ELISA达1∶32;间接ELISA的精密性(变异系数CV)为6.98%,竞争ELISA为5.10%。结论建立了用间接ELISA检测抗狂犬病毒IgG抗体和竞争ELISA检测抗狂犬病毒总抗体的方法,并生产出相应试剂盒。Objective Establishing the methods of detecting the rabies virus IgG antibody with the indirect ELISA and the total antibody with competitive ELISA. Methods Rabies virus antigen was prepared with Vero-cell infected by RV-CTN, which was concentrated and purrificated, and indirect ELISA and competitive ELISA were established. Results Quality appraisal showed that the specificity accord ratio of the 10 positive and 20 negative samples, was 100% ; and the sensibility of indirect ELISA was 1： 640, the competitive ELISA was 1： 32 ; the preciseness （ Variability Coefficient, CV） of the indirect ELISA was 6.98%, and the competitive ELISA was 5.10%. Conclusion This research established successfully the methods of detecting the rabies virus IgG antibody with the indirect ELISA and the total antibody with competive ELISA, and the reagent cases were produced.

关 键 词：狂犬病毒 间接ELISA 竞争ELISA IGG抗体 总抗体 

分 类 号：R512.99[医药卫生—内科学]