HPLC法测定人血浆中盐酸特拉唑嗪的浓度  

Determination of Terazosin Hydrochloride in Human Plasma by HPLC

作　　者：丁翔宇[1] 蒋晔[1] 安普丽[1] 郝福[1] 李艳荣[1] 

机构地区：[1]河北医科大学药学院,石家庄市050017

出　　处：《中国药房》2007年第14期1076-1078,共3页China Pharmacy

摘　　要：目的:建立以反相高效液相色谱法测定人血浆中盐酸特拉唑嗪浓度的方法。方法:血样采用二氯甲烷-乙醚(2:3)提取后测定,其中色谱柱为Diamonsil C18,流动相为0·05mol·L-1(pH4·8)磷酸二氢钾缓冲液-甲醇-乙腈(61:25:14),流速为1·0mL·min-1,荧光检测器激发波长为334nm,发射波长为385nm。结果:盐酸特拉唑嗪检测浓度在0·25～64ng·mL-1范围内线性关系良好(r=0·9995),最低检测限为0·1ng·mL-1;平均提取回收率为84·0%,平均方法回收率为100·8%;日内RSD≤3·4,日间RSD≤3·0。结论:本方法简便、准确、灵敏,可用于人血浆中盐酸特拉唑嗪的临床药动学研究和血药浓度检测。OBJECTIVE： To establish a RP- HPLC method for the determination of terazosin hydrochloride in human plasma.METHODS：Terazosin hydrochloride and internal standard （parazosin hydrochloride） were extracted from plasma with dichlormethane- diethylether（2 ： 3） for determination.The separation was carried on a Diamonsil Cls column with the mobile phase consisted of 0.05mol · L^-1 potassium dihydrogen phosphate buffer solution（pH4.8） -methanol- acetonitrile（61 ： 25 ： 14） .The flow rate of the mobile phase was 1.0mL · min^-1,and the excitation and emission wavelengths of the florescence detector were set at 334 and 385 nm, respectively.RESULTS：The linear range of terazosin hydrochloride was 0.25-64ng · mL^-1 （r = 0.999 5）, with the lowest detectable limit at 0. lng · mL^-1.The average extraction recovery was 84.0% and the average methodological recovery was 100.8% .The intra-day RSD and inter- day RSD were no more than 3.4% and 3.0%, espectively.CONCLUSION：This method is rapid, simple, accurate and sensitive, and suitable for the pharmacokinetic study and assaying of terazosin hydrochloride.

关 键 词：高效液相色谱法 盐酸特拉唑嗪 血药浓度 荧光检测器 

分 类 号：R969.1[医药卫生—药理学]