钙影响花生胚胎发育/败育特异蛋白质的筛选与鉴定  

Isolation and Identification of Specific Expressed Proteins from Peanut(Arachis hypogaea L.) Development /Abortion Embryo Mediated by Calcium

作　　者：张君诚[1] 蔡宁波[2] 张新文[1] 庄伟建[2] 

机构地区：[1]三明学院应用生物技术研究所,福建三明365004 [2]福建农林大学作物学院油料研究所,福建福州350002

出　　处：《作物学报》2007年第5期814-819,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30070481);福建省三明市科技局资助项目(2003-C-3)

摘　　要：花生(Arachis hypogaea L.)缺钙造成严重空荚(胚胎败育),导致减产、降质,长期困扰南方花生生产。以大田足(施足钙)、缺(缺钙地大田本底)钙条件下花生的种植与观察为基础,利用改良的传统双向电泳、MALDI-TOF质谱和蛋白质数据库等手段,得到7个与受钙影响花生早期胚胎败育/发育有关的差异表达蛋白。4#差异蛋白是与菠菜(Spinaciaoleracea)的70 kD的叶绿体被膜休克相关蛋白高度相似的缺钙组表达上调的特异蛋白;6#差异蛋白与三叶胶(Heveabrasiliensis)的线粒体前体中ATP合酶的β链高度相似,该蛋白在缺钙幼胚缺失;7#差异蛋白在低钙组缺失,其与马铃薯(Solanum tuberosum)的肌动蛋白97或100类似。研究结果提示,受钙胁迫花生幼胚败育可能与早期幼胚内能量转化、氧化还原系统、细胞内膜以及细胞骨架功能维持相关的功能基因的表达异常有关。初步揭示了受钙影响花生胚胎败育的分子机理。Empty pods and ill-filled pods by calcium deficient has been a long existing problem in peanut production in the red and yellow soil regions in the southern China. Based on the measurements and comparisons in the field experiments with different levels of calcium, the seven differentially expressed proteins which related to the development/abortion embryos of peanut were got by 2-D electrophoresis, MALDI-TOF-MS and protein databases. The results searched from the protein databases were as follows： 4^# protein, which up-regulated in embryos from deficient calcium group, showed high homology with the 70 kD protein from the chloroplast envelope membrane of Spinacia oleracea L. ; 6^# protein disappeared in embryos from deficient calcium group showed high homology with the β-chain of the （ATPB） ATP synthase from Hevea brasiliensis L. ; and 7^# protein also disappeared in embryos from deficient calcium group was considered similar to the Actin 97＆100 from Solanum tuberosum. All the results indicated that the molecular mechanism of embryo abortion mediated by calcium may be relevant to some genes controlling energy transferring（ ATP synthesis）, oxidoreduction system, endo-membrane system and cytoskeleton structure system which are transcripted or expressed unnomally.

关 键 词：胚发育 胚败育 钙 蛋白质 花生(Arachis HYPOGAEA L.) 

分 类 号：S565.2[农业科学—作物学]