碱性磷酸酶在体外钙化中的作用  

THE ROLE OF ALKALINE PHOSPHATASE IN THE PROCESS OF CALCITICATION IN VITRO

作　　者：刘侠 乐以伦 

出　　处：《四川大学学报（自然科学版）》1997年第1期96-99,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：报道了用碱性磷酸酶（ＡＫＰ）抑制剂左旋咪唑和缓钙化剂偕二磷酸钠（Ｎａ２ＥＨＤＰ），通过体外钙化实验，跟踪测试ＡＫＰ比活力及４５Ｃａ在佝偻病大鼠的骺骨生长板中的掺入．结果表明，钙化在酶失活、缺乏Ｃａ２＋或Ｐｉ的条件下不会发生，ＡＰＫ活力对钙化的发生是必需的，ＡＫＰ活力越高，钙化速度越快，同时，钙化会加速ＡＫＰ失活，钙化速度越快。Growth plate cortilage of rachitic rat was used to studied factors influencing biological calcification.Levamisole,a inhibitor of alkaline phosphatase (AKP) and disodium ethanehydroxydiphosphonate (Na 2EHDP),a anticalcification agent,were used to study the velation between AKP and calafication.Measurements of specific activity of AKP and 45 Ca uptuke by rachitic cartilage slices from metastable calcium phosphate solution indicated that heat and without Ca 2+ or Pi in the solution inhibited calcification almost totally,and levamisole and Na 2EHDP inhibited calcification partily.The results suggest that AKP is essential for biologic calcification.In the process of biologic calcification,The acticity of AKP will be loss gradually,The faster the speed of calcification is,the faster the loss of AKP activity is.

关 键 词：体外钙化 碱性磷酸酶 钙化作用 

分 类 号：Q555.402[生物学—生物化学]