小麦耐盐相关基因TaSTK的克隆  

Cloning of Salt-Tolerance Related Gene TaSTK in Triticum aestivum

作　　者：葛荣朝[1] 赵宝存[1] 陈桂平[2] 秘彩莉[1] 沈银柱[1] 黄占景[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]唐山师范学院生物科学技术系,河北唐山063000

出　　处：《作物学报》2007年第5期857-860,共4页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30070471);河北省自然科学基金项目(C2005000164)

摘　　要：利用RACE方法,从小麦耐盐突变体RH8706-49中扩增获得小麦丝氨酸/苏氨酸蛋白激酶(Triticum aestivumserine-threonine protein kinase)基因TaSTK的全长cDNA序列,含1 958 bp,其中开放阅读框1 431 bp,编码476个氨基酸。其编码区基因组DNA全长为4 095 bp,含5个外显子。该基因的全长cDNA序列及基因组序列均已提交GenBank数据库(登录号:DQ103756和DQ341377)。经过NCBI比对发现该基因的氨基酸序列与丝氨酸/苏氨酸蛋白激酶、酪氨酸蛋白激酶、蛋白激酶都具有较高的同源性。Northern杂交检测表明,TaSTK在小麦中属于盐诱导增强型基因,并且在耐盐材料RH8706-49中诱导增强程度高于敏盐材料H8706-34。TaSTK的杂交信号非常弱,表明TaSTK在小麦幼苗的叶片组织中属于低表达的基因类型。The full-length cDNA sequence of TaSTK gene contained 1 958 bp was obtained from the salt-resistant wheat （ Triticum aestivum） mutant RH8706-49 by RACE method. The coding sequence was 1 431 bp and by which 476 amino acids were encoded. The genome sequence of coding location contained 4 095 bp and 5 extrons. The cDNA sequence and genome sequence of TaSTK gene were submitted to GenBank （Accession No. QD103756, DQ341377）. According to the BLAST results, the peptide encoded by this gene showed high homology to serine/threonine protein kinases （S_TKc, SPS1）, tyrosine kinase （TyrKc） and protein kinase （Pkinase）. Northern blotting results showed that TaSTK was a saltinduced gene in wheat. Under salt stress, the expression of TaSTK was elevated more strongly in the salt-tolerant line RH8706-49 than in the salt-sensitive line H8706-34. At the same time, the hybridization signals were all very weak, indicating that TaSTK belongs to the low expression gene in the wheat seedling tissue.

关 键 词：耐盐基因 小麦 丝氨酸 苏氨酸蛋白激酶 CDNA末端快速扩增 基因克隆 

分 类 号：S512.1[农业科学—作物学]