迷迭香酸抗穿透支原体脂蛋白诱导巨噬细胞凋亡的作用研究  

Effect of Rosmarinic Acid against the RAW264.7 Cell Apoptosis Induced by LAMPs of Mpe

作　　者：迟秀玲[1] 张海强[1] 钟超[1] 吴移谋[2] 朱炳阳[1] 游晓星[2] 严奉祥[1] 

机构地区：[1]南华大学药物药理研究所,衡阳421001 [2]南华大学病原微生物研究所,衡阳421001

出　　处：《现代生物医学进展》2007年第4期525-528,共4页Progress in Modern Biomedicine

基　　金：湖南省卫生厅基金(204062);湖南省教育厅重点基金(03A040);国家教育部重点基金(204095);国家"十五"攻关项目(02EFN214300457)

摘　　要：探讨迷迭香酸对穿透支原体脂蛋白诱导巨噬细胞凋亡的保护作用。方法:以穿透支原体脂蛋白损伤RAW264．7细胞为模型,用MTT法检测细胞存活状况,DNA片断化分析观察穿透支原体脂蛋白对RAW264．7细胞DNA降解的影响,碘化丙啶染色流式细胞术检测细胞凋亡率。结果:7．5mg/L穿透支原体脂蛋白可引起RAW264．7细胞存活率降低,出现细胞凋亡特征性“DNA梯带”,流式细胞术检测细胞出现凋亡亚G1期峰,凋亡率为31．9%;100μmol/L迷迭香酸预处理1小时后可以升高RAW264．7细胞的存活率,凋亡细胞特征性的梯状梯带消失,细胞凋亡亚G1期峰消失,并使RAW264．7细胞的凋亡率下降为7．9%。结论:迷迭香酸有抗穿透支原体脂蛋白诱导RAW264．7细胞凋亡的作用。Objective： To investigate the protected effect of RosA against RAW264.7 cells apoptosis induced by lipidassociated membrane proteins （LAMPs） of Mycoplasma penetrans （Mpe）. Methods：With the model of RAW264.7 cells apoptosis induced by LAMPs of Mpe, the live viability of RAW264.7 cells being treated 24 hours by LAMPs of Mpe was first observed by MTT assay; then the changes of RAW264.7 cells DNA degradation were observed by DNA fragmentation analysis; and the rate of RAW264.7 cells apoptosis was detected by flow cytometric analysis. Results：Different concentrations of LAMPs of Mpe could obviously inhibit growth and the survival rate of RAW264.7 cells, while 7.5mg/L LAMPs of Mpe treated for 24 hours could make the survival rate of RAW264.7 cells close to IC50. DNA fragmentation analysis showed up the DNA ladder of typical apoptotic feature after 7.5mg/L LAMPs of Mpe treated for 24 hours. Result of flow cytometrlc analysis showed the appearing of the second G1 period peak of apoptosis and the rate of apoptosis was up to 31,9% after the same treatment as before, while the cell apoptosis rate of normal group was 2.84%. After one hour＇s pretreatment of different concentrations of Ros A, we could detected by MTT assay that survival rate of RAW264.7 cells was obviously increased, while pretreated by 100μmol/L Ros A and it showed the best protected effect on RAW264.7 cells. DNA ladder of typical apoptotic feature was not detected, and the second G1 period peak of cells apoptosis was lost under this condition. The apoptotic rate of RAW264.7 cells was decreased obviously from 31.9% to 7.9%. Conclusions：Ros A has the function of inhibiting the apoptosis of RAW264.7 cells induced by LAMPs of Mpe.

关 键 词：迷迭香酸 穿透支原体LAMPs RAW264.7细胞 抗凋亡 

分 类 号：Q255[生物学—细胞生物学]