人HT036蛋白的原核表达及鉴定  

Prokaryotic expression and identification of human HT036 protein

作　　者：彭旭[1] 袁顺宗[1] 王晓娟[1] 甘成军[1] 王庆红[1] 陈烯伟[1] 张小容[1] 胡晓红[1] 罗高兴[1] 吴军[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所创伤,烧伤与复合伤国家重点实验室,疾病蛋白质组学重庆市市级重点实验室,重庆400038

出　　处：《第三军医大学学报》2007年第9期817-819,共3页Journal of Third Military Medical University

摘　　要：目的原核表达人HT036蛋白并鉴定其正确性。方法采用PCR技术,扩增人HT036蛋白编码序列,克隆至原核表达载体pET30a(+)中,用IPTG诱导表达,免疫印迹鉴定。结果PCR扩增得到了目的DNA片段,克隆至原核表达载体后经酶切与测序鉴定正确,并在大肠杆菌中获得高效表达,用抗His抗体鉴定融合蛋白表达正确。结论成功构建了重组原核表达载体并正确表达了HT036蛋白。Objective To express human HT036 protein in Escherichia coli （E. coli. ） and identify it. Methods The eDNA sequence obtained by PCR was cloned into the prokaryotie expression plasmid pET30a （ ＋ ）. The target protein was expressed in E. coll., induced by IPTG and analyzed by Western blotting. Results The interest gene was identified by restriction endonucleases digestion and DNA sequencing. The protein was highly expressed in E. coll.. Conclusion We successfully expressed the HT036 protein.

关 键 词：HT036蛋白 原核表达 增生性瘢痕 

分 类 号：Q503[生物学—生物化学] Q511