补脾方药对脾虚大鼠壁细胞内三磷酸肌醇(IP_3)含量的影响  

Effect of Pi-invigorating recipe and herbs on Inositol 1,4,5-Triphosphate (IP_3) in parietal cells of Pi-deficiency rats

作　　者：李燕舞[1,2] 王汝俊[1,2] 崔琦珍[1,2] 王建华[1,2] 陈芝喜[1,2] 梁雪芬[1,2] 

机构地区：[1]广州中医药大学脾胃研究所 [2]广州中医药大学核医学室,广州510405

出　　处：《中药药理与临床》2007年第2期1-2,共2页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(NO30271650)

摘　　要：目的:观察脾虚大鼠模型壁细胞内IP3含量的变化及补脾方药党参、白术、补中益气汤对其的影响。方法:大鼠壁细胞采用EDTA-Pronase酶消化法,采用竞争蛋白结合法测定大鼠壁细胞内IP3含量。结果:大黄脾虚模型大鼠壁细胞内IP3含量显著低于正常组,经党参、白术、补中益气汤治疗后,IP3含量有不同程度的升高,尤以党参组明显。结论:脾虚证可能与壁细胞内IP含量生成量减少相关,补脾方药对其有一定的调节作用。To observe the changes of IP3 in parietal cells of Pi - deficiency rats and the effects of medicines for invigorating Pi. Methods： The Parietal cells isolated from rats were made by Pronase - EDTA method. The IP3 assay is based on competition between ^3H - IP3 and unlabelled IP3 in the standard or samples for binding to a binding protein prepared from bovine adrenal cortex. Results： The contents of IP3 in parietal cells of Pi - deficiency rats obviously decreased. After treatment by Dangshen（党参）, Baizhu（白术） and Buzhong Yiqi decoction （补中益气汤）, the contents of IP3 in parietal cells increased. Conclusions： The decreased synthesis of IP3 in parietal cells perhaps related with the condition of Pi - deficiency of rats. And the mechanism of medicines for invigorating Pi might partly act through the regulation of the intracellular messenger.

关 键 词：脾虚证 壁细胞 IP3 党参 白术 补中益气汤 

分 类 号：R285.5[医药卫生—中药学]