金丝桃苷对鸭乙肝病毒感染的保肝作用  

Liver protective effects of hyperin on duck hepatitis B virus infection

作　　者：鲁小杰[1] 黄正明[1,2] 杨新波[3] 汪晖[1] 耿淼[3] 李壮[4] 

机构地区：[1]武汉大学医学部药理学系,武汉430072 [2]解放军302医院药学部,北京100039 [3]解放军301医院老年医学研究所,北京100853 [4]中国医学科学院医药生物技术研究所病毒室,北京100050

出　　处：《中药药理与临床》2007年第2期10-12,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金;编号30572350

摘　　要：目的:观测金丝桃苷(Hyp)对鸭乙肝病毒(DHBV)感染所致肝损伤的保护作用。方法:一日龄北京雏鸭接种DHBV阳性血清,7d后灌胃给药10日,停药后第3天检测肝匀浆和血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)及血清中总胆红素(TBil)、白蛋白(ALB)、碱性磷酸酶(ALP)、胆碱酯酶(ChE)和乳酸脱氢酶(LDH)的含量,另行肝组织病理学检查。结果:模型组血中TBil、ALP、LDH和ALB及肝匀浆中ALT和AST活力显著升高,肝脏病理改变明显,肝索紊乱,肝细胞桥接坏死,汇管区大量炎细胞浸润。Hyp组血清中TBil和ALP及肝匀浆ALT均显著降低,肝病理改变较轻。结论:雏鸭感染DHBV模型可见肝细胞严重受损,肝功能异常,肝脏和血中各种酶含量明显升高。Hyp对DHBV感染所致雏鸭肝损伤有较好的保护作用。Objective： To investigate biochemical changes in serum and liver homogenate of ducklings due to duck hepatitis B virus （DHBV） infection; and to investigate the effects of hyperin（Hyp） on these changes. Method： One day old Peking ducklings were divided into 7 groups randomly： normal control group, model control group, Hyp low-dose group （15 mg/kg.d）, Hyp medium-dose group （30 mg/kg.d）, Hyp high-dose group（60 mg/kg.d）, Lamivudinevir group（50 mg/kg.d） and Entecavir group（0.25 mg/kg.d）. AU but ducklings in normal control group were infected with DHBV positive serum. Intragastric administration started 7 days later, bid for 10 days successively. Animals in normal and model control groups were administrated with proportional amount of normal saline. Animals were sacrificed 3 days after the last administration. Alanine aminotransferase（ALT） and aspartate aminotransferase （AST） in liver homogenate as well as ALT, AST, total bilirubin（ Tbil）, albumin（ ALB）, alkaline phosphatase（ ALP）, cholinesterase（ ChE）, and lactate dehydrogenase（LDH） in serum of each animal were assayed. A small portion of liver tissue from each liver sample was fixated by formalin for routine HE staining. Result： Compared with normal control, serum contents or activities of TBil, ALP, LDH and ALB of model group were significantly high, so were ALT and AST activity of liver homogenate of model group; severe liver pathological changes were observed in model group, with derangements of hepatic cord, bridging necrosis and regeneration of hepatocytes, and hyperplasia of fibrocyte. After Hyp treatment, serum content of TBil and serum activity of ALP decreased significantly, so did the ALT activity of liver homogenate, moreover, liver pathological changes were greatly ameliorated. Conclusion： DHBV infection leads to hepatocyte injury, decreased liver functions in secretion and excretion, and bile excretion blockage. Hyp is partly effective in protecting ducklings from liver injuries resulted f

关 键 词：金丝桃苷 DHBV 肝损伤 

分 类 号：R285.5[医药卫生—中药学]