糖尿病大鼠阴茎海绵体组织中核因子κB表达的研究  

Study of transcription factor nuclear factor-κB in cavernous tissue of rat models of diabetes mellitus

作　　者：李燕[1] 王扬天[2] 姚乐申[3] 陈岽[3] 戴玉田[3] 孙则禹[3] 

机构地区：[1]南京中医药大学第二附属医院内科,江苏南京210017 [2]南京军区南京总医院内分泌科,江苏南京210002 [3]南京大学医学院附属鼓楼医院泌尿外科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2007年第4期380-384,F0004,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省"135"工程重点医学人才基金资助(RC2001002)

摘　　要：目的:观察糖尿病雄性SD大鼠阴茎海绵体组织中核因子-κB(NF-κB)活性的变化以及分布,探讨糖尿病性勃起功能障碍(ED)发病机制。方法:将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法(EMSA)进行NF-κB活性检测。结果:B组、C组大鼠勃起功能水平较A组显著降低(P<0.05),其海绵体组织中NF-κB活性显著高于A组(P<0.05),且NF-κB表达随病程延长呈现逐渐增强趋势;糖尿病性大鼠阴茎海绵体组织中NF-κB主要分布在内皮细胞中,平滑肌细胞和其他细胞中分布较少。结论:糖尿病大鼠阴茎海绵体组织中NF-κB活性及分布与正常相比有明显差异,且与糖尿病病变程度有密切关系,提示NF-κB在糖尿病性ED发病中可能起重要作用。Objective：To investigate the mechanism of diabetic erectile dysfunction （ED） by detecting the variation of the activity and distribution of nuclear factor-kappaB （NF-κB） in the corpus cavernosum of rats with diabetes mellitus. Methods：All of 40 male Sprague-Dawley rats were divided into 3 groups：control group（group A, n = 10）, diabetic group for 4 weeks（group B, n = 14）, and diabetic group for 12 weeks（group C, n = 16）. The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay （EMSA）. Results：Compared with those in group A,erection function was down-modulated significantly, and the activity of NF-κB in penile tissues was higher in group B and group C （P 〈 0.05）. And the expression of NF-κB had an increasing tendency with the extension of the disease course. NF-κB was mainly expressed in the endothelial cell of the corpus cavernosum, however less in contractile fiber cells and other cells. Conclusion：The activity and distribution of NF-κB in the corpus cavernosum of diabetic rats has been shown to be altered with diabetes. NF-κB might play an important role in the develoment of diabetic ED.

关 键 词：糖尿病 勃起功能障碍 NF-κB 阴茎海绵体 大鼠 

分 类 号：R587[医药卫生—内分泌]