从COⅠ和COⅡ基因部分序列研究中国翠凤蝶亚属(鳞翅目:凤蝶科)的分子系统关系(英文)  

Phylogeny of Princeps butterflies from China: evidences from mitochondrial partial COⅠ and COⅡ gene sequences (Lepidoptera: Papilionidae)

作　　者：诸立新[1] 吴孝兵[1] 晏鹏[1] 武春生[2] 

机构地区：[1]安徽师范大学生命科学学院 [2]中国科学院动物研究所,北京100080

出　　处：《动物学报》2007年第2期257-263,共7页ACTA ZOOLOGICA SINICA

基　　金：This research was funded by the grants fromthe Special Fund for Anhui Young Leaders Fellowship (No.04043049) ;Anhui Scholar Special Fund inScience and Technology;the Key Laboratory of Biotic Environment and Ecological safetyin Anhui Province

摘　　要：为了探讨中国尾凤蝶翠凤蝶亚属(Princeps)的系统发生关系,本文对中国产10种翠凤蝶亚属蝴蝶的细胞色素氧化酶Ⅰ(COⅠ)基因(约627bp)、细胞色素氧化酶Ⅱ(COⅡ)基因部分序列(约690bp)进行了分析。在获得的1317个位点中,有184个变异位点,144个简约信息位点;表现出明显的A+T含量(74.2%)偏倚。采用MP、ML法构建了分子系统树,结果显示:达摩翠凤蝶(P.demoleus)、巴黎翠凤蝶(P.paris)可能是中国最早起源的翠凤蝶亚属的蝴蝶,与其它翠凤蝶亚属蝴蝶的亲缘关系较远;碧翠凤蝶(P.bianor)、波绿翠凤蝶(P.polyctor)、穹翠凤蝶(P.dialis)和短尾翠凤蝶(P.doddsi)的亲缘关系较近;窄斑翠凤蝶(P.arcturus)、克里翠凤蝶(P.krishna)、绿带翠凤蝶(P.maackii)和西番翠凤蝶(P.syfanius)的亲缘关系较近。结果同时表明:短尾翠凤蝶和穹翠凤蝶没有序列差异,碧翠凤蝶和波绿翠凤蝶序列差异为0.15%,因此短尾翠凤蝶是穹翠凤蝶的同物异名。In order to study phylogenetic relationships among Princeps species of China, partial CO Ⅰ （about 627 bp） and CO Ⅱ （about 690 bp） gene sequence from 10 species of Princeps in China were analyzed. There were 184 variable sites and 144 parsimony-informative sites in the 1 317 nucleotides of the data, Princeps exhibited a 74.2% Arginine and Thymine bias. Molecular phylogenetic trees were reconstructed using maximum parsimony （MP） method and maximum likelihood （ML） method. The molecular phylogenetic trees showed that P. demoleus, P. paris perhaps was the basal to other Princeps species from China. For P. bianor, P.polyctor, P. dialis and P. doddsi, results suggested that relationships among them were closer. The relationships among P. arcturus, P. Krishna, P. maackii and P. syfanius were also closer. There was no sequence divergence between CO Ⅰ and CO Ⅱ gene sequences of P, dialis and P. doddsi, and the sequence divergence between P. bianor and P.polyctor was 0.15%. The results indicated that P. doddsi should be treated as synonym of P. dialis, and P.polyctor should be treated as synonym of P. bianor .

关 键 词：翠凤蝶亚属 COⅠ基因 COⅡ基因 系统发生关系 

分 类 号：Q961[生物学—昆虫学] Q963