壳聚糖对急性氨氮胁迫下中华鳖稚鳖非特异性免疫反应的影响  

The effect of chitosan on some non-specific immune indices of the juvenile soft-shelled turtle Pelodiscus sinensis

作　　者：宋超[1,2] 牛翠娟[1] 陈欣然[1] 

机构地区：[1]北京师范大学生命科学学院,生物多样性与生态工程教育部重点实验室,北京100875 [2]北京市辐射安全技术中心

出　　处：《动物学报》2007年第2期270-277,共8页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助(No.30271014)~~

摘　　要：本研究旨在探讨壳聚糖对急性氨氮胁迫下中华鳖(Pelodiscus sinensis)稚鳖非特异性免疫功能的影响。实验共设5组,实验组中华鳖分别腹腔注射0mg/ml(C0组)、1mg/ml(C1组)、5mg/ml(C2组)、50mg/ml(C3组)壳聚糖溶液0.1ml,并饲养于总氨氮浓度(TAN)为110mg/L的水环境中;对照组注射等量生理盐水溶液并饲养于新鲜晾晒自来水中。注射7d后观测外周血和脾脏中淋巴细胞ANAE阳性率、血清旁路途径溶血活性、血清溶菌活性。与对照组相比,C0组外周血T淋巴细胞ANAE阳性率和血清旁路途径溶血活性均有显著提高,脾脏T淋巴细胞和血清溶菌活性没有显著变化。与C0组相比,注射壳聚糖各组外周血T淋巴细胞ANAE阳性率和血清溶血活性均有不同程度的显著下降,并且恢复到接近对照组的水平(P>0.05),只有C3组溶血活性显著低于对照组;注射壳聚糖对脾脏中T淋巴细胞ANAE活性没有显著影响。C2组溶菌活性显著高于其他组,其他各组间差异不显著。表明壳聚糖对氨氮胁迫导致的免疫应激反应有一定的拮抗作用。This study was conduced to investigate the effect of chitosan on non-specific immune function in juvenile soft-shelled turtles under acute ammonia stress. Turtles were divided into five groups. Turtles in the treatment groups were injected with 0 mg/ ml （CO group）, lmg/ml （C1 group）, 5 mg/ml （C2 group）, or 50 mg/ml （C3 group） chitosan solution 0.1 ml, and exposed to ammonia stress with 110 mg/L total ammonia nitrogen （TAN） in their aquaria. The control group was injected with the same volum of physiological salt solution and reared in fresh water. After 7 days, we measured a-naphthyl acetate esterase （ANAE） activity in peripheral blood and spleen, hemolytic activity and bactefiolytic activity. CO group showed higher ANAE positivity in blood and hemolytic activity than the control, but showed no significant difference in bactefiolytic and ANAE activity in spleen. ANAE positivity in blood and hemolytic activity decreased after injecting with chitosan in the ammonia-stressed groups and resumed to the control level （ P 〉 0.05 ）. Only the C3 group showed significantly lower hemolytic activity than the control. The injection of chitosan induced no significant change in ANAE positivity in spleen. Bactefiolytic activity in the C2 group was higher than in the other groups. In conclusion, chitosan could restore the stress responses induced by ammonia exposure. The degree of this effect depended on the concentration of chitosan solution and the immune indices tested.

关 键 词：中华鳖 壳聚糖 氨氮胁迫 ANAE阳性率 血清旁路途径溶血活性 溶菌活性 

分 类 号：S917.4[农业科学—水产科学]