5-氨基酮戊酸光动力学疗法抗单纯疱疹病毒的实验研究  

Experimental studies on treatment of HSV infections with photodynamic therapy using 5-aminolevnlinic acid

作　　者：陶纪宁 段淑敏[2] 李军 

机构地区：[1]上海复旦张江生物医药股份有限公司,201203 [2]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华实验和临床病毒学杂志》2007年第1期79-82,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：上海市科技发展基金项目（01DJGK012）

摘　　要：目的 研究5-氨基酮戊酸光动力学疗法（ALA-PDT）在体内外抗单纯疱疹病毒（HSV）的作用。方法 以HSV-Ⅰ感染豚鼠皮肤的动物模型以及HSV-Ⅰ和HSV-Ⅱ感染的Vero细胞为实验系统，观察ALA-PDT抗单纯疱疹病毒的疗效。结果 ALA-PDT在体外具有抑制HSV-Ⅰ和HSV-Ⅱ的作用，与阳性药物无环鸟苷作用基本一致。体内实验中，外用ALA对HSV-Ⅰ型豚鼠皮肤感染仅进行一次PDT治疗，对皮肤病变就产生了剂量依赖性的治疗效果，中、高剂量组疗效显著。结论 ALA-PDT能够有效地灭活单纯疱疹病毒，治疗该病毒引发的皮肤病变。为该疗法用于病毒性疾病的治疗提供了依据。Objective To investigate in vitro and in vovo anti-human herpes simplex virus effects of photodynamic therapy （PDT） using 5-aminolevulinic acid （ALA）. Methods Guinea pigs model of cutaneous herpes virus infection was applied, and Vero cells infected by HSV-Ⅰ and HSV-Ⅱ were used as experimental systems to observe the antiherpes effect of ALA-PDT.Results The in vitro experiments showed that ALA-PDT has antiherpes effect on HSV-Ⅰ and HSV-Ⅱ, its effect was similar to that of acyelovir. The results of animal experiments showed that ALA-PDT had significant therapeutic effect on guinea pigs model of cutaneous herpes virus infection, the effect was dose-related. Conclution ALA-PDT could be effective in treating HSV infections, which may provide a new approach to the treatment of viral infections.

关 键 词：光化学疗法 氨基酮戊酸 单纯疱疹病毒属 豚鼠 

分 类 号：R686[医药卫生—骨科学]