我国汉坦病毒的基因进化与起源的分析  

Analysis of hantaviruses origin and molecular evolution in China

作　　者：赵晋丰[1] 曹广文[1] 

机构地区：[1]第二军医大学卫生勤务学系流行病学教研室,上海200433

出　　处：《第二军医大学学报》2007年第4期360-363,共4页Academic Journal of Second Military Medical University

基　　金：军队医学杰出人才基金(04J08);军队"十一五"科技攻关项目(06G05)~~

摘　　要：目的:推测汉坦病毒在我国的起源时间和地点。方法:从GenBank中获得部分在我国分离的汉坦病毒M片段序列,未及登录的序列通过联系文献报道的作者获得。用MEGA3．1程序对这些序列进行比对并做种系发生分析。用同一地区间隔5年以上分离的同一亚型病毒株对作为姐妹序列推算汉坦病毒在我国的进化速率,结合各病毒株的分离时间和地点计算其在我国的起源时间和地点。结果和结论:汉坦病毒的地理分布具有聚集性．贵州地区的亚型最多,支持关于我国汉坦病毒起源于贵州省的推断。从安徽地区发现了一种可能为新型的病毒．提示安徽地区可能容易促进汉坦病毒发生变异。Gou3株与祖先病毒株分离最早,推测我国汉坦病毒可能起源于315年前左右。Objective： To investigate the first appearance and geographic distribution of hantaviruses in China. Methods： Some sequences of M segment of hantaviruses isolated in China were retrieved from GenBank. Some recently sequenced viruses which have not been registered in GenBank were obtained from authors who reported them. Program MEGA 3. 1 was employed for the phylogenetic analysis of the above virus sequences. Nucleotide divergence rates were estimated by comparing the closely related sister sequences isolated in the same geographical region at no less than a 5-year interval. Based on these calculated divergence rates, together with their isolating time and region, we explored the first appearance and geographic distribution of hantaviruses in China. Results and conclusion： The distribution of hantaviruses was in cluster, with Guizhou Province having the most subgenotypes, which supports the previous hypothesis that hantaviruses first appeared in Guizhou Province of China. A novel subgenotype virus was found in Anhui Province, indicating that it might be easier for the virus to have mutation in Anhui Province. We also found that Gou3 strain might be the earliest one to separate from a common ancestral strain; it is speculated that hantaviruses migrated in China about 315 years ago.

关 键 词：汉坦病毒 进化 分子 地理分布 起源时间 

分 类 号：R373.32[医药卫生—病原生物学]