热应激对大鼠肝组织及人前列腺癌PC-3细胞RhoB表达的影响  

Effect of heat stress on expression of small G protein RhoB in rat livers and human prostate cancer cell line PC-3 cells

作　　者：刘亚萍[1] 李忆东[1] 王晓慧[1] 杨瑞[1] 卢建[1] 

机构地区：[1]第二军医大学基础部病理生理学教研室,上海200433

出　　处：《第二军医大学学报》2007年第4期381-384,共4页Academic Journal of Second Military Medical University

摘　　要：目的:观察烫伤后大鼠肝脏组织及热应激后前列腺癌PC-3细胞小G蛋白RhoB表达的变化,探讨热应激对体内外RhoB表达的影响。方法:制备雄性SD大鼠30％体表面积Ⅲ度背部烫伤模型,烫伤后2、4、8、16 h取相应大鼠肝脏组织(n= 6),应用RT-PCR和Western印迹分析测定RhoB mRNA和蛋白水平,并以正常大鼠肝脏组织作对照(n=6)。制备人前列腺癌PC-3细胞热应激模型,热应激后0．5、1、2、4、8 h取细胞检测RhoB的表达,并以未处理细胞作对照。结果:烫伤后4 h,大鼠肝组织RhoB mRNA表达量最高,为正常对照的3．2倍(P<0．01),8 h开始下降;而RhoB蛋白表达量在烫伤后8 h最高(P<0．01)。PC-3细胞RhoB mRNA在热应激后2 h时开始上调;4 h时达到最大值,约为对照的2．8倍(P<0．01);热应激后8 h RhoB蛋白表达量最高(P<0．01)。结论:在体内、体外实验中热应激均能上调小G蛋白RhoB mRNA和蛋白的表达。Objective：To investigate the changes of the small G protein RhoB expression in scalded rat livers and heatstressed human prostate cancer cell line PC-3, so as to discuss the influence of heat stress on expression of RhoB in vitro and in vivo. Methods.. Third degree burns of 30% total body surface area （TBSA） model was established with SD rats on the back. The expression of RhoB mRNA and protein in the liver was determined by RT-PCR and Western blot 2 h, 4 h, 8 h and 16 h （n=6） after scalding; the liver tissues of normal rats were taken as control（n=6）. PC-3 cells were allowed to recover for 0.5 h, 1 h, 2 h, 4 h and 8 h in a cellular heat stress model and the expression of RhoB in mRNA and protein were determined by RT-PCR and Western blot, respectively; untreated PC-3 cells were taken as control. Results： The expression of RhoB mRNA in the livers peaked 4 h after scalding, being about 3.2 folds that of the control group（P〈0.01） ; the expression began to decline 8 h after scalding. The expression of RhoB protein peaked 8 h after scalding, significantly higher than that of the control （P〈0.01）. RhoB mRNA level began to increase 2 h after heat stress treatment and peaked at 4 h, being about 2.8 folds that of the control（P〈0. 01）. The expression of RhoB protein reached its maximum at 8 h after heat stress treatment（P〈0.01）. Conclusion： Heat stress can up-regulate the expression of RhoB mRNA and protein in vivo and in vitro.

关 键 词：热应激 烫伤 小G蛋白 RHOB 肝 前列腺肿瘤 

分 类 号：R644[医药卫生—外科学]