GDNF及不同生长底物对培养颈上神经节交感神经元的作用  

Effects of GDNF and substrate on superior cervical ganglion sympathetic neurons in vitro

作　　者：文灿[1] 李淑蓉[2] 张艳玲[1] 王晗知[1] 苏炳银[1] 

机构地区：[1]第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所重庆400038 [2]成都医学院病理学教研室,成都610081

出　　处：《第三军医大学学报》2007年第10期882-884,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目("973"项目);(2003CB515300);成都医学院科研专项基金(2006)~~

摘　　要：目的探讨胶质细胞源性神经营养因子及不同生长底物对培养颈上神经节交感神经元存活和突起生长的影响。方法取新生大鼠颈上神经节(superior cervical ganglion,SCG)体外分离培养,培养基中含不同浓度的胶质细胞源性神经营养因子(glial cell derived neurotrophic factor,GDNF),并选取不同方法溶解多聚-D-赖氨酸(poly-D-lysine,PDL)包被培养盖片,观测交感神经元的存活和突起生长状况。结果用0.01mol/L硼酸溶解的PDL进行包被,细胞存活和突起生长最好,且神经元分散好,优于其他各组;GDNF对培养交感神经元的存活、突起生长具有显著的促进作用,且与其浓度存在量效关系,但它并不能阻止培养前5d存活神经元数量的减少。结论用0.01mol/L硼酸溶解PDL包被是一种良好的生长底物处理方法,GDNF能促进离体培养的交感神经元的存活和突起生长。Objective To explore the effects of glial cell-derived neurotrophic factor and different substrates on cell survival and neurite growth of superior cervical vitro. Methods Sympathetic neurons from superior cervical ganglion sympathetic neurons from neonatal rat in ganglion （SCG） of neonatal rat respectively were dissociated and cultured. The glial cell-derived neurotrophic factor （GDNF） of different quantities were added in the medium, and culture cover slips were coated by variant methods with poly-D-lysine （PDL）. The cell survival and neurite growth of cultured sympathetic neurons were observed by phase-contrast microscopy. Results In the group of cover glasses coated with PDL dissolved by 0.01 mol/L boric acid solution, ceils grew dispersedly survival well, and both the cell survival and neurite growth were better than others. GDNF promoted the cell and neurite growth of sympathetic neurons markedly, and there was a dose-effect relationship, but it could not abolish the decrease of survival cells from 1 d to 5 d in vitro. Conclusion It is a favorable substrate that coated with PDL dissolved 0.01 mol/L by boric acid solution, and both the cell survival and neurite growth of sympathetic neurons can be improved by GDNF in vitro.

关 键 词：胶质细胞源性神经营养因子 生长底物 交感神经元 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R329-33[医药卫生—基础医学]