人尿路上皮细胞TLR2和TLR4分布情况研究  

Distribution of Toll-like receptor 2 and 4 in human urinary tract

作　　者：徐华超[1] 卢根生[1] 张庆华[2] 

机构地区：[1]第三军医大学西南医院全军泌尿外科中心,重庆400038 [2]解放军150医院泌尿外科,洛阳471000

出　　处：《第三军医大学学报》2007年第10期948-950,共3页Journal of Third Military Medical University

摘　　要：目的探讨Toll样受体(Toll-like receptor,TLR)2和4在人尿路上皮细胞中的表达情况。方法体外培养人肾小管、肾盂、输尿管和膀胱上皮细胞,提取总RNA,采用半定量逆转录-聚合酶链反应(RT-PCR)测定不同部位的含量。结果肾小管、肾盂、输尿管和膀胱上皮细胞TLR2的相对表达量分别为0.326、0.589、0.602、0.735,而TLR4的相对表达量分别为0.223、0.327、0.379、0.365,所有部位尿路上皮细胞中TLR2含量显著高于TLR4(P<0.05),肾小管上皮细胞TLR2、TLR4含量显著低于其余各组(P<0.05)。结论人尿路上皮细胞中TLR2和TLR4均有表达,尿路感染致病菌分布量可能与TLR亚型表达情况有关。Objective To study the mRNA expression of Toll-like receptor （TLR） 2 and 4 in human urinary tract epithelium. Methods The epithelial cells of nephric tubule, pelvis, ureter and bladder were cultured in vitro, and their mRNA expressions of TLR2 and TLR4 were measured by RT-PCR. Results The mRNA expression of TLR2 in nephric tubule, pelvis, ureter and bladder was 0.326, 0. 589, 0. 602 and 0.735, and that of TLR4 was 0.223, 0.327, 0. 379, 0.365, respectively. The mean TLR2 mRNA copy was significantly higher than TLR4 in each tissue （P 〈 0. 05 ）. The expression of TLR2 and TLR4 in nephric tubule was significantly lower than other tissues （ P 〈 0.05 ）. Collusion That TLR2 and TLR4 both expressed in human urinary tract epithelium is probably associated with the distribution of uropathogenic bacteria.

关 键 词：上皮细胞 TOLL样受体 尿路感染 

分 类 号：R322.6[医药卫生—人体解剖和组织胚胎学] R345[医药卫生—基础医学]