小鼠肾小球系膜细胞的增龄性改变及油酸对其影响  

The aging-induced pathophysiological changes of glomerular mesangial cells in mice and the effect of oleic acid on the changes

作　　者：程庆砾[1] 赵佳慧[1] 张冬梅[1] 张晓英[1] 

机构地区：[1]解放军总医院老年心肾科,北京市100853

出　　处：《中华老年医学杂志》2007年第4期293-295,共3页Chinese Journal of Geriatrics

摘　　要：目的探讨C57BL／6J小鼠肾小球系膜细胞增龄性改变以及油酸对系膜细胞的影响。方法实验动物采用28月龄、3月龄小鼠，测定小鼠血清肿瘤坏死因子α（TNFα）水平，检测系膜细胞的增殖状况，测定肾小球系膜细胞培养上清中胶原Ⅰ、胶原Ⅳ和基质金属蛋白酶2（MMP-2）的含量，观察油酸对系膜细胞的影响。结果28月龄小鼠与3月龄小鼠比较，血清TNFα水平明显增高，分别为（71．6±17．3）μg／L和（59．7±9．7）μg／L，差异有统计学意义（P〈0．05）；肾小球系膜细胞增殖能力明显增强；细胞培养上清中胶原Ⅰ[（52．1±4．6）ng／1万个细胞对（20．3±3．5）ng／1万个细胞]、胶原Ⅳ[（26．3±3．5）ng／1万个细胞对（7．6±1．3）ng／1万个细胞]的水平明显增高；随着鼠龄的增加，细胞培养上清中MMP-2的活性明显下降。油酸可以刺激小鼠肾小球系膜细胞的增殖和胶原Ⅳ的分泌，尤以28月龄小鼠为著。结论与3月龄小鼠比较，28月龄小鼠的肾小球系膜细胞出现明显硬化病变倾向，油酸可刺激28月龄小鼠肾小球系膜细胞的增殖及胶原的分泌。Objective To investigation of the effects of oleic acid （OA） on the aging-induced pathophysiological changes of glomerular mesangial cells （GMCs） in mice. Methods The glomeruli were isolated from 28 months-age-old mice （aged mice） and 3 month-age-old mice （young mice） respectively using the microdissection and the GMCs were cultured. The concentration of serum TNF-α was detected by ELISA. The proliferation of GMCs was analyzed using [^3H]-thymidine uptake and the concentration of collagen type Ⅰ or type Ⅳ in cell supernatant were assayed by ELISA. The gelatinase activity （MMP-2） in the conditioned medium was determined by zymography. Results （1）The serum TNFα in aged mice was higher than that in young mice; （2）ThepH-lthymidine incorporation of GMCs in aged mice was markedly increased than that in young mice, and the concentration-dependent increase of [^3H]-thymidine incorporation was induced by addition of OA within 10 to 100 μmol/L; （3）The level of collagen type Ⅳ in the GMCs cultured medium in aged mice was higher than that in young mice, and OA induced an increase of collagen type Ⅳ in the cell supernatant. However, there was no difference in the level of collagen type Ⅰ between the aged and young mice; （4）The MMP-2 activity of conditioned medium were markedly decreased in the aged mice. Conclusions The oleic acid can induced a concentration-dependent increase of mitogenic response and collagen type Ⅳ secretion in the GMCs of the aged mice. Oleic acid may be is a risk factor for glomerular mesangial sclerosis in the aged mice.

关 键 词：肾小球系膜 油酸 肿瘤坏死因子Α 胶原 

分 类 号：R686[医药卫生—骨科学]