大鼠原位肝移植模型技术的若干改进  

Improvements on Surgical Model Techniques of Rat Orthotopic Liver Transplantation

作　　者：王谦[1] 李立[1] 冉江华[1] 李晓延[1] 陈刚[1] 闫宏宪[1] 

机构地区：[1]昆明市第一人民医院暨昆明医学院临床学院肝脏移植中心,云南昆明650011

出　　处：《昆明医学院学报》2007年第2期43-45,共3页Journal of Kunming Medical College

摘　　要：目的大鼠原位肝移植的模型技术的改进.方法采用远交系SD和远交系Wistar大鼠,用二袖套法(门静脉和肝下下腔静脉)建立大鼠原位肝移植模型,正式实验60次.结果术中尾静脉建立输液通路,改进了供肝的修剪和受体肝上腔静脉的阻断与吻合方法,修肝平均时间8 min,受体无肝期平均21 min,手术成功率达85%.结论改进的大鼠原位肝移植术操作简便,手术成功率较高,可做为肝移植实验可靠、稳定的动物模型.Objective To improve some surgical model techniques of rat orthotopic liver transplantation. Methods Two -cuff （portal vein and infrahepatic vena cava） technique was applied to the production of the animal model in SD and Wistar rats. Results With the improvements a successful rate of 85% was achieved in the production of the experimental model with anhepatic period of an average 21 minutes. Conclusion The results show that the model is stable and can be used in the experiment of liver transplantation in the rats.

关 键 词：大鼠 外科 手术 肝移植 

分 类 号：R657.3[医药卫生—外科学]