霉酚酸酯对糖尿病大鼠肾脏的保护作用  

Protective effect of mycophenolate mofetil to renal tissures in diabetic rats

作　　者：黄晓丽[1] 吕永曼[2] 罗建峰 易艳[2] 

机构地区：[1]武汉市第一医院肾内科,湖北武汉430022 [2]华中科技大学同济医学院同济医院肾内科,湖北武汉430030 [3]武汉市新洲区人民医院急诊科,湖北武汉430400

出　　处：《基础医学与临床》2007年第4期421-425,共5页Basic and Clinical Medicine

摘　　要：目的探讨霉酚酸酯(MMF)对糖尿病模型大鼠肾脏的保护作用及其机制。方法SD大鼠随机分为3组:对照组、糖尿病模型组和糖尿病治疗组。治疗12周后,检测大鼠血糖(BG)、血尿素氮(BUN)、血肌酐(Scr)、肾肥大指数(肾重KW/体重BW)、肌酐清除率(Ccr)和24h尿蛋白(24Upro)。肾组织做常规光镜和电镜检查。用免疫组织化学方法检测肾组织中细胞间黏附分子-1(ICAM-1)和增殖细胞核抗原(PCNA)蛋白的表达,半定量RT-PCR的方法检测肾组织中ICAM-1mRNA的表达。结果与对照组相比,糖尿病组大鼠BG、KW/BW、24Upro、BUN、Scr和Ccr均显著升高(P<0.05或0.01);系膜密度和肾小球基底膜厚度均显著增加(P<0.01);肾组织内ICAM-1和PCNA的蛋白质表达及ICAM-1的mRNA表达均显著上调(P<0.05或0.01)。除血糖外,治疗组上述指标均显著回降(P<0.05或0.01)。结论MMF对糖尿病肾病有保护作用,其机制可能与下调ICAM-1和PCNA的表达有关。Objective To assess the renal protective effects of mycophenolate mofetil （MMF） in diabetic model rats and to explore its mechanism. Methods SD rats were divided randomly into there groups： control group, diabetic model group and diabetic group treated with MMF. In 12 weeks, blood glucose（ BG）, blood urea nitrogen（ BUN）, serum creatinine（ Scr）, index number of kidney hypertrophy （kidey weight to body weight, KW/BW）, creatinine clearance（Ccr） and 24- hour urinary protein （24Upro） were detected. Kidney tissure were examined by microscopy. The protein exprssion of intercellular adhesion molecule-1 （ICAM-1） and proliferating cell nuclear antigen （PCNA） in renal tissure were determined by immunohistochemical technique. The mRNA expression of ICAM-1 in kidney tissure were semi-quantitatively determined with reverse transcription-polymerase chain reaction（RT-PCR）. Results Compared with control group, BG,KW/BW,24Upro,BUN ,Scr and Ccr were significantly increased in diabetic model rats（P 〈0. 05 or 0. 01 ）, the density of mesangium and the depth of glomerular basement membrane were significantly enlarged（P 〈0. 01 ）, the protein expression of ICAM-1 and PCNA and the mRNA expression of ICAM-1 were significantly up-regulated （P〈0. 05 or 0.01 ）. Except blood glucose, the above mentioned parameters in MMF treated group were all significantly inhibited （P 〈 0.05 or 0. 01 ）. Conclusion MMF has protective effect to diabetic nephropathy. The mechanism may be correlated with that it down-regulate the exprssion of ICAM- 1 and PCNA.

关 键 词：霉酚酸酯 糖尿病肾病 细胞间黏附分子-1 增殖细胞核抗原 

分 类 号：R587.2[医药卫生—内分泌]