缺血预处理对大鼠减体积肝移植后Ref-1蛋白表达的影响  

The effects of ischemia precondition on the expression of Ref-1 protein after reduced-size rat liver transplantation

作　　者：刘现忠[1] 姚爱华[1] 仲跻巍[1] 张长和[1] 李相成[1] 

机构地区：[1]南京医科大学第一附属医院肝脏移植中心,210029

出　　处：《中华普通外科杂志》2007年第4期250-253,共4页Chinese Journal of General Surgery

基　　金：江苏省省科委优秀青年基金资助项目（BQ2002012）

摘　　要：目的 观察缺血预处理（ischemia preconditioning，IPC）对大鼠减体积肝移植术后氧化还原因子-1（redox factor-1，Ref-1）蛋白表达的影响，以探讨IPC对减体积肝移植损伤的保护作用及其机制。方法 将100只Lewis成年雄性大鼠随机分成三组：IPC移植组（IPC组）、50％减体积肝移植组（PLT组）和假手术组（SO组），分别于移植后0．5h、2h、6h和24h取材，通过Western blot法和免疫组织化学技术结合图像分析定量检测移植后各时间点Ref-1表达变化，同时结合血清学和组织病理学分析Ref-1表达变化的意义。结果 术后PLT组各时间点血清ALT值分别为（595．06±108．78）U／L；（723．18±117．24）U／L；（1186．65±142．31）U／L；（1498．91±126．79）U／L；IPC组术后各时间点血清ALT值分别为（459．06±84．73）U／L；（587．71±95．23）U／L；（799．61±125．97）U／L：（659．27±135．68）U／L。与PLT组相比，IPC组术后6h和24h血清ALT值明显降低（t=4．553，P〈0．05；t=10．110，P〈0．01）。病理学分析显示，PLT组术后24h门静脉周围大量炎细胞浸润，肝窦扩张明显，肝组织损伤较重；而IPC组则损伤较轻。与PLT组相比，IPC组于减体积肝移植术后24h肝实质细胞中Ref-1蛋白表达明显增高。结论 缺血预处理可以减轻减体积肝移植后早期肝脏损伤，促进肝组织Ref-1蛋白表达，提示缺血预处理保护减体积肝移植物早期损伤的机制可能与促进Ref-1蛋白的表达有关。Objective To observe the effects of ischemia preconditioning （IPC） on the expression of Ref-1 protein after reduced-size liver transplantation in rats and explore the mechanisms by which IPC protects against liver injury. Methods One hundred adult male Lewis rats were randomly divided into three groups： 50% sized liver transplantation （PLT group ）, 50% liver transplantation with ischemia preconditioning （IPC group） and sham operation group （SO group）. Animals were sacrificed in each group at different time points： 0. 5 h,2 h,6 h and 24 h after liver transplantation. Then, we explore the changes and significance of the expression of Ref-1 according to Western blotting and immunohistochemistry, in combination with serology and histopathology analysis. Results The serum ALT value of animal in PLT group for 0.5 h,2 h,6 h and 24 h after liver transplantation was （595.06 ± 108.78） U/L; （723.18 ± 117.24） U/L; （1186.65 ± 142.31） U/L; （1498.91 ± 126.79） U/L; and （459.06 ± 84.73） U/L; （587.71 ± 95. 23） U/L;（799. 61 ± 125.97 ） U/L; （659. 27 ± 135. 68） U/L for IPC group. As compared with PLT group, the ALT value at 6 h and 24 h after operation in IPC group decreased significantly （ t = 4. 553,P 〈 0. 05;t = 10. 110, P 〈 0. 01 ）. By pathobiological analysis, we found that there were lots of inflammation cells infiltrating the portal venulae, significant sinus hepaticus dilation and damage of hepatic parenchyma. The damage in IPC group was comparatively slight. There was an increased expression of Ref-1 protein in both IPC group and PLT group compared with SO group. The expression of Ref-1 protein in IPC group was higher than that in PLT group at 24 h after reduced-size liver transplantation. Conclusions Ischemia preconditioning relieves the liver injury after reduced-size liver transplantation during early period, and facilitates the expression of Ref-1 protein in hepatic tissue after transplantation, implying the protection mechanism is at least partially rel

关 键 词：肝移植 再灌注损伤 缺血预处理 氧化还原因子1 

分 类 号：R686[医药卫生—骨科学]