西伯利亚蓼rd22基因的克隆与序列分析  

Cloning and Sequence Analysis of rd22 Gene from Polygonum sibricum

作　　者：郑磊[1] 刘关君[1] 杨传平[1] 

机构地区：[1]东北林业大学林木遗传育种省级重点实验室,哈尔滨150040

出　　处：《植物研究》2007年第2期212-217,共6页Bulletin of Botanical Research

基　　金：国家重大基础研究发展规划973项目(G1999016003)

摘　　要：以3%NaHCO3溶液胁迫处理48h的西伯利亚蓼为试材,利用RACE技术,从其茎部组织克隆了脱水应答蛋白RD22的全长cDNA序列。测序后的结果分析表明,该cDNA序列全长为1302bp,5′非翻译区为59bp,3′非翻译区为25bp,开放读码框为1218bp,编码405个氨基酸。在氨基酸序列的C端含有一个比较保守的BURP结构域,N端含有5个重复序列THV-VGKGGV-V。信号肽检测证明该蛋白为分泌性蛋白,前21个氨基酸区域为信号肽结构。其推演的氨基酸序列与葡萄的同源性最高,达到60%。该基因已在GenBank上注册,基因序列登录号为DQ836050。Using RACE （rapid amplification of cDNA ends） method, the full-length cDNA of dehydration-responsive protein RD22 was cloned from Polygonum sibricum treated with 3% NaHCO3 solution for 48. The sequence analysis shows that the rd22 gene is 1 302 bp in length, including 59 bp of 5＇untranslated region, 25 bp of 3＇untranslated region and 1 218 bp of open reading frame （ORF）. The rd22 gene encodes a protein of 405 amino acids. The C-terminal of the RD22 protein contains a conserved BURP domain, and the N-terminal contains 5 copies of a repetitive sequence of THV-VGKGGV-V. Signal peptide prediction shows the RD22 protein is a secretory protein, and has a signal peptide structure at the first 21 amino acids region. The amino acid sequence of RD22 prutein from Polygonum sibricum has a high homology with grape of 60%. This rd22 gene has been accepted by GenBank, and the accession number of gene sequence is DQ836050.

关 键 词：西伯利亚蓼 rd22基因 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]