激光辅助制动对精子核DNA的影响  

Laser-assisted Immobilization Causes No Firect Damage to Sperm DNA

作　　者：徐志鹏[1] 孙海翔[1] 胡娅莉[1] 张宁媛[1] 招霞[1] 

机构地区：[1]南京大学医学院附属鼓楼医院生殖医学中心,江苏南京210008

出　　处：《中华男科学杂志》2007年第3期216-218,共3页National Journal of Andrology

基　　金：南京市科技局重点基金(20028013)

摘　　要：目的:研究激光辅助制动精子是否引起精子核DNA损伤。方法:23例行体外受精(IVF)的男性精液标本,上游法处理后用0.45ms脉冲激光照射精子尾部制动精子。采用末端转移酶介导的dUTP末端标记法(TUNEL)和单细胞凝胶电泳法(SCGE)两种方法分别检测激光辅助制动处理前后阳性精子百分率。结果:TUNEL法检测激光辅助制动处理前后阳性精子的百分率分别为(1.32±0.61)%、(1.41±0.51)%,差异无显著性(P>0.05);SCGE法检测处理前后阳性精子百分率分别为(1.59±0.70)%、(1.83±0.68)%,差异亦无显著性(P>0.05)。结论:激光照射精子尾部辅助制动精子对精子核DNA无明显损伤。Objective： To determine whether laser-assisted immobilization of sperm damages sperm DNA. Methods： Twenty-three semen samples were selected from an IVF program. Then normal spermatozoa were obtained by swimming-up method and immobilized with the tail by 0. 45 ms pulse laser. Terminal deoxynucleotidys transferase mediated dUTP nick end labeling （TUNEL） and single cell gel electrophoresis （SCGE） were used to detect sperm DNA damage. Results： There was no significant difference either before and after laser treatment in the percentage of TUNEL-positive spermatozoa （ [ 1.32 ±0.61 ] % vs [ 1.41 ±0.51 ] % , P 〉 0.05 ） or in SCGE （ [ 1.59 ±0.70]% vs [ 1.83 ±0.68]%, P〉0.05）. Conclusion： Laser-assisted sperm immobilization may cause no direct damage to the sperm DNA.

关 键 词：精子制动 激光 DNA损伤 末端转移酶介导的dUTP末端标记法 单细胞凝胶电泳 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]