中药红花浸液对植入期子宫内膜白血病抑制因子表达水平的影响  

The effect of Chinese-drug-saffron steep on the expression of leukemia inhibitory factor in the endometrium of implantation

作　　者：廉瑞青[1] 马保华[1] 王哲民[2] 

机构地区：[1]山东大学医学院组织学与胚胎学教研室,济南250012 [2]山东省中医药研究所生物化学室

出　　处：《现代妇产科进展》2007年第3期194-196,I0001,共4页Progress in Obstetrics and Gynecology

基　　金：山东省自然科学基金资助课题(No:Y2004C08)

摘　　要：目的:探讨中药红花抗早孕过程中的生物学作用机理。方法:随机将受精后第2天的孕鼠分为3个实验组和对照组,分别经口给予不同浓度的红花液和生理盐水,连续3天,停药后24天取子宫,石蜡包埋切片,进行HE和免疫组化染色,将染色结果输入图像分析仪,观察子宫内膜的形态和白血病抑制因子(leukemia inhibitory factor,LIF)蛋白的表达,并作定量分析。结果:实验组子宫内膜形态有异常改变;免疫组化染色表明,随着药物剂量的增多,子宫内膜细胞LIF蛋白的表达逐渐减少;但对照组呈强阳性表达。结论:中药红花作用于孕鼠子宫内膜细胞,抑制LIF蛋白的表达,从而影响胚胎着床,起到了抗早孕的作用。Objective：To study the biological mechanism of Chinese-drug-saffron during anti-early pregnancy. Method：The mated female mice were divided into three experimental groups,saffon with different concentrations were used on the 2nd day after fertilization, which lasting for 3 days, and the change of the morphology endometrium was observed. The sections were stained with HE and LIF immunohistochemistry, quantitative analysis was proformed. Resuit- In experimental groups, the endometrium morphology changed significantly and the expression of LIF-protein decreased gradually with the increasing of concentration, whereas the control group stable positive LIF-protein expression. Conclusion：The Chinese-drug-saffron can inhibit the expression of LIF-protein on the endometium and may have a role of anti-early-pregnancy.

关 键 词：红花 白血病抑制因子 子宫内膜 抗早孕 

分 类 号：R714.12[医药卫生—妇产科学]