急性心肌梗死患者应用粒-巨噬细胞集落刺激因子后血CD34^+细胞和炎性因子的变化  

Changes of the peripheral CD34 positive cells and inflammatory factors levels after using GM-CSF in patients with acute myocardial infarction

作　　者：邓争荣[1] 马爱群[1] 杨春[1] 陈新义[2] 

机构地区：[1]西安交通大学医学院第一附属医院心内科环境与疾病相关基因教育部重点实验室,陕西西安710061 [2]陕西省人民医院心内科,陕西西安710068

出　　处：《西安交通大学学报（医学版）》2007年第2期152-155,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的观察急性心肌梗死患者应用粒-巨噬细胞集落刺激因子(GM-CSF)后,外周血CD34+细胞的变化,探讨GM-CSF对炎性因子的影响。方法20例急性心肌梗死患者被随机分为GM-CSF[10μg/(kg.d),共7 d]组和对照组(生理盐水)。应用流式细胞仪检测患者外周血CD34+细胞的绝对计数。应用酶联免疫法及放射免疫法检测两组患者及10例健康者血C-反应蛋白(CRP)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的水平。结果与对照组相比,GM-CSF组在应用GM-CSF后外周血CD34+细胞数明显增高,于第7天达到高峰(P<0.05);心肌梗死患者血CRP、IL-6及TNF-α水平均明显高于健康者(P<0.05);GM-CSF组与对照组相比,CRP水平从第3天时开始升高,第10天时较对照组有明显的差别(P<0.05);两心梗组在IL-6及TNF-α水平上均无统计学意义的差别。结论GM-CSF可有效的动员急性心肌梗死患者外周血CD34+细胞,亦可增加CRP的水平。Objective To investigate the changes of the peripheral CD34 positive cells in patients with acute myocardial infarction （AMI） after using granulocyte-macrophage colony-stimulating factor （GM-CSF） and to discuss the effects of GM-CSF on inflammatory factors. Methods Twenty patients with AMI were randomly divided into GM-CSF group （10μg/kg body weight, for seven days） and control group （saline）. The absolute counts of CD34 positive cells in peripheral blood were enumerated with flow cytometry. Plasma levels of C-reactive protein （CRP）, interleukin-6 （IL-6）, and tumor necrotic factor- α （TNF- α） were measured with enzyme-linked immunoassay and radioimmunoassay. Results Peripheral CD34 positive cells increased shortly after using GM-CSF and peaked on day 7 compared with those of controls （P〈0.05）. The levels of the CRP, IL-6 and TNF- α in patients with AMI were significantly greater than those in the healthy （P〈0. 05）. The plasma levels of CRP in GM-CSF group inclined to increase from day 3, but only on day 10 GM-CSF group had significantly higher levels of plasma CRP than controls （P〈0. 05）. The levels of IL-6 and TNF- α in the treated patients were the same as those in controls. Conclusion GM-CSF can effectively mobilize the CD34 positive cells and may increase the level of plasma CRP in patients with AMI.

关 键 词：急性心肌梗死 GM—CSF CD34^+细胞 CRP 

分 类 号：R542.22[医药卫生—心血管疾病]