窄谱中波紫外线对角质形成细胞肝素结合表皮生长因子样生长因子mRNA的影响  

Effects of narrow-band UVB in inducing the expression of heparin-binding epidermal-growth-factor-like growth factor mRNA in normal human keratinocytes

作　　者：罗素菊[1] 彭振辉[1] 郑焱[1] 张路坤[1] 王国荣[2] 徐浩翔[1] 

机构地区：[1]西安交通大学医学院第二附属医院皮肤科,陕西西安710004 [2]陕西省人民医院普通外科,陕西西安710068

出　　处：《西安交通大学学报（医学版）》2007年第2期211-214,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30371295;30400387);陕西省自然科学基金资助项目(No.2004C211)

摘　　要：目的研究窄谱中波紫外线(NB-UVB)对培养的正常人角质形成细胞(KC)增殖和肝素结合表皮生长因子样生长因子(HB-EGF)mRNA表达的影响。方法用50 mJ/cm2和100 mJ/cm2NB-UVB照射正常人KC后继续培养12 h,用MTT法和实时荧光定量RT-PCR法分别检测KC增殖和HB-EGF mRNA的改变。结果NB-UVB照射正常人KC后,可剂量依赖抑制KC的增殖和上调HB-EGF mRNA的表达。50 mJ/cm2和100 mJ/cm2NB-UVB照射后,分别使KC增殖抑制5.4%和8.7%,使HB-EGF mRNA增加到正常对照组的1.7倍和2.5倍。结论NB-UVB抑制KC的增殖部分是通过上调HB-EGF的表达介导的。Objective To investigate the alteration of cell proliferation and heparin-binding epidermalgrowth-factor-like growth factor （HB-EGF）mRNA expression in cultured normal human keratinocytes after NB-UVB irradiation. Methods After irradiation with 50 mJ/cm^2 and 100 mJ/cm^2 NB-UVB in normal human keratinocytes, the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of HB-EGF mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results Both keratinocytes growth inhibition and HB-EGF mRNA expression were dose-dependently induced by NB-UVB. The proliferation of keratinocytes was inhibited by 5.4% and 8.7%, and the expression of HB-EGF mRNA was elevated by 1.7- and 2.5-fold after irradiation with 50 mJ/cm^2 and 100 mJ/cm^2 NB-UVB, respectively. Conclusion The growth inhibition induced by NB-UVB in normal human keratinocytes may be mediated at least in part by upregulated HB-EGF expression.

关 键 词：表皮生长因子 角质形成细胞 窄谱中波紫外线 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]