大鼠下丘脑KiSS-1与GnRH mRNA的表达  

Expression of KiSS-1 and GnRH in Rat Hypothalamus

作　　者：王雪松[1] 张才田[1] 居晓斌[1] 张朝晖[1] 丁贵鹏[1] 陈子庆[1] 苏恩本[1] 崔毓桂[1] 

机构地区：[1]南京医科大学第一附属医院生殖医学中心,江苏南京210029

出　　处：《中华男科学杂志》2007年第4期315-319,共5页National Journal of Andrology

基　　金：江苏省135医学重点人才项目(RC2002076)

摘　　要：目的:观察不同发育阶段雄性大鼠下丘脑KiSS-1和GnRH mRNA表达,探讨KiSS-1在性发育的启动和正常生殖功能调节中的作用。方法:雄性SD大鼠分为幼年期(12d)、未发育期(20d)、发育早期(35d)、性发育期(45d)和性成熟期(75d)共5组,每组6只,分离下丘脑,采用RT-PCR和实时定量-PCR的方法检测各发育阶段大鼠下丘脑KiSS-1和GnRH mRNA的水平。化学发光法检测各发育阶段大鼠血清睾酮水平。结果:大鼠随发育过程KiSS-1 mRNA转录水平逐渐升高,到性发育期水平最高,成年期略下降,但保持相对较高水平。未发育期、发育早期、性发育期和性成熟期大鼠KiSS-1 mRNA水平分别是幼年期的1.7、2.1、3.5、2.0倍。GnRH与KiSS-1表达变化呈正相关(r=0.905,P<0.05),GnRH激活晚于KiSS-1。GnRH在性发育期转录水平最高,未发育期、发育早期、性发育期和性成熟期表达水平分别是幼年期的1.1、1.9、2.4、1.9倍。大鼠在性发育期睾酮水平较发育前显著升高,性成熟期水平最高。结论:KiSS-1表达与GnRH表达具有正相关性,其可能参与GnRH的调控,与青春期启动和生殖功能调节有关。Objective： To assay the expression of KISS-1 and GnRH in the male rat hypothalamus at different developmental stages, and to explore the significance of KISS-1 in sex development onset and normal reproduction regulation. Methods： Expression analyses of KISS-1 and GnRH genes were conducted in the rat hypothalamus at different developmental stages with RT-PCR and real time-PCR. The testosterone level was assayed by chemoluminescenee technique. Results： KISS-1 mRNA rose gradually during sex development in the rat hypothalamus, highest at puberty and lowered a little at adulthood. KISS-1 mRNA of the prepubertal, early pubertal, pubertal and adult rats was 1.7, 2.1, 3.5 and 2.0 times higher than that of the infantile rats respectively. The expression of GnRH and KISS-1 correlated positively （ r = 0.905,P 〈 0.05）. But the activation of GnRH neuron was later than KISS-1. The expression of GnRH was the highest in the puberty rats. GnRH mRNA of the prepubertal, early pubertal, pubertal and adult rats was 1.1, 1.94, 2.42 and 1.92 times higher than that of the infantile rats respectively. The level of testosterone in the adult rats was significantly higher than that at the earlier stage and was the highest at the adult stage. Conclusion ： The expression of KISS-1 correlates positively with that of GnBH. KISS-1 may participate in the regulation of GnRH and is relevant to puberty onset and the regulation of reproduction function.

关 键 词：KISS-1 促性腺激素释放激素 性发育 大鼠 

分 类 号：Q42[生物学—神经生物学]